Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, PO Box: 14155-6153, Tehran,Iran.
Comb Chem High Throughput Screen. 2022;25(2):252-258. doi: 10.2174/1386207323666201230091042.
The genus Thymus is one of the well-known members of the Lamiaceae, comprising about 215 species. It is a polymorphic genus in this family. Therefore, possible differences in chemical composition among polymorphs may result in differences in their biological activities.
This study was designed to specify chemical composition in order to assess antioxidant activities and to characterize active constituents of essential oil of Thymus pubescens.
Essential oil was collected by hydrodistillation and was analyzed using GC-MS and GCFID. Antioxidant activities were evaluated by ABTS radical cation scavenging and β-carotene bleaching inhibition methods. Detection and identification of antioxidant components were performed by TLC-bioautography assay using ABTS•+ reagent.
GC analyses indicated the presence of 39 components, accounting for 96.64% of total constituents, with thymol (38.67%), γ-terpinene (7.46%), and p-cymene (5.54%) as the main components. The IC50 values for antioxidant activities were equal to 1.15 (1.05 - 1.25) μg/mL and 0.45 (0.33 - 0.62) μg/mL for ABTS•+ scavenging and β-carotene bleaching inhibition tests, respectively. TLC-bioautography of oil resulted in the identification of thymol as the major antioxidant compound.
Essential oil exhibited very potent and significant antioxidant activities correlated well with increasing dose in vitro.
百里香属是唇形科中众所周知的成员之一，约有 215 种。它是该科中一个多态性的属。因此，多态种之间的化学成分可能存在差异，这可能导致它们的生物活性存在差异。
本研究旨在明确化学成分，以评估抗氧化活性，并对绒毛百里香精油的活性成分进行表征。
通过水蒸馏法收集精油，并使用 GC-MS 和 GCFID 进行分析。通过 ABTS 自由基清除和β-胡萝卜素漂白抑制法评估抗氧化活性。使用 ABTS•+试剂通过 TLC-生物自显影法检测和鉴定抗氧化成分。
GC 分析表明，存在 39 种成分，占总成分的 96.64%，主要成分是百里酚（38.67%）、γ-萜品烯（7.46%）和对伞花烃（5.54%）。抗氧化活性的 IC50 值分别为 1.15（1.05-1.25）μg/mL 和 0.45（0.33-0.62）μg/mL，用于 ABTS•+清除和β-胡萝卜素漂白抑制试验。油的 TLC-生物自显影导致鉴定出百里酚是主要的抗氧化化合物。
精油表现出非常强的、显著的抗氧化活性，与体外的剂量增加呈正相关。
