Faedmaleki Firouz, H Shirazi Farshad, Salarian Amir-Ahmad, Ahmadi Ashtiani Hamidreza, Rastegar Hossein
Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2014 Winter;13(1):235-42.
Nano-silver (AgNP) has biological properties which are significant for consumer products, food technology, textiles and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles are largely used in various commercially available products. Thus, the use of nano-silver is becoming more and more widespread in medicine. In this study we investigated the cytotoxic effects of AgNPs on liver primary cells of mice, as well as the human liver HepG2 cell. Cell viability was examined with MTT assay after HepG2 cells exposure to AgNPs at 1, 2, 3, 4, 5, 7.5, 10 ppm compared to mice primary liver cells at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration-dependent decrease of cell viability in both cells. IC50 value of 2.764 ppm (µg/mL) was calculated in HepG2 cell line and IC50 value of 121.7 ppm (µg/mL) was calculated in primary liver cells of mice. The results of this experiment indicated that silver nanoparticles had cytotoxic effects on HepG2 cell line and primary liver cells of mice. The results illustrated that nano-silver had 44 times stronger inhibitory effect on the growth of cancerous cells (HepG2 cell line) compared to the normal cells (primary liver cells of mice). which might further justify AgNPs as a cytotoxic agents and a potential anticancer candidate which needs further studies in this regard.
纳米银（AgNP）具有对消费品、食品技术、纺织品和医学应用（如伤口护理产品、可植入医疗器械、诊断、药物递送和成像）具有重要意义的生物学特性。由于其抗菌活性，银纳米颗粒广泛用于各种市售产品。因此，纳米银在医学中的应用越来越广泛。在本研究中，我们研究了AgNP对小鼠肝脏原代细胞以及人肝癌HepG2细胞的细胞毒性作用。在HepG2细胞暴露于1、2、3、4、5、7.5、10 ppm的AgNP后，与暴露于1、10、50、100、150、200、400 ppm的小鼠原代肝细胞24小时相比，用MTT法检测细胞活力。AgNP在两种细胞中均导致细胞活力呈浓度依赖性下降。在HepG2细胞系中计算出的IC50值为2.764 ppm（μg/mL），在小鼠原代肝细胞中计算出的IC50值为121.7 ppm（μg/mL）。该实验结果表明，银纳米颗粒对HepG2细胞系和小鼠原代肝细胞具有细胞毒性作用。结果表明，与正常细胞（小鼠原代肝细胞）相比，纳米银对癌细胞（HepG2细胞系）生长的抑制作用强44倍。这可能进一步证明AgNP作为一种细胞毒性剂和潜在的抗癌候选物，在这方面需要进一步研究。
