Khoshneviszadeh Mahsima, Ashkani-Esfahani Soheil, Namazi Mohammad Reza, Noorafshan Ali, Geramizadeh Bita, Miri Ramin
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Pharm Res. 2014 Winter;13(1):263-9.
Wounds and wound healing have always been one of the most important subjects that experimental researches were dedicated to. Simvastatin has been used for long as a common lipid lowering agent which was reported to have some pleiotropic effects such as antioxidation, anti-inflammation and immunomodulation. In this study we aimed to determine the effect of simvastatin on wound healing process in laboratory rats by means of stereological and histopathological analyses. 36 male Sprague-Dawley rats (220 ± 20 g) with a 1 cm(2) circular full-thickness wound on their back were divided into three groups: SS group that received a gel with 2% concentration of simvastatin; UW group that received no treatment but daily irrigation with normal saline; Base group that was treated with the gel base. Duration of the study was 12 days and at the end, wound closure rate, grade of inflammation, granulation-tissue formation, ulceration, epithelization, fibroblast proliferation, collagen-bundles synthesis, and vascularization were determined. Outcome of this study revealed that Simvastatin improves the wound healing by its anti-inflammatory and epithelization induction effect as well as statistically significant induction of fibroblast proliferation and collagen bundle synthesis which were reported by our stereological and histopathological investigations. Results of the present study demonstrated that topical Simvastatin enhances the wound healing process through affecting different aspects of tissue regeneration; however, further researches are needed to find the exact mechanism, advantages and disadvantages of this chemical agent.
伤口与伤口愈合一直是实验研究致力于探索的最重要课题之一。辛伐他汀长期以来一直作为一种常用的降脂药物使用，据报道它具有一些多效性作用，如抗氧化、抗炎和免疫调节作用。在本研究中，我们旨在通过体视学和组织病理学分析来确定辛伐他汀对实验大鼠伤口愈合过程的影响。36只背部有1平方厘米圆形全层伤口的雄性Sprague-Dawley大鼠（220±20克）被分为三组：SS组，接受含2%浓度辛伐他汀的凝胶治疗；UW组，不接受治疗但每天用生理盐水冲洗；基础组，用凝胶基质治疗。研究持续时间为12天，最后测定伤口闭合率、炎症程度、肉芽组织形成、溃疡、上皮化、成纤维细胞增殖、胶原束合成和血管化情况。本研究结果显示，辛伐他汀通过其抗炎和诱导上皮化作用，以及我们的体视学和组织病理学研究所报道的对成纤维细胞增殖和胶原束合成的统计学显著诱导作用，改善了伤口愈合。本研究结果表明，局部应用辛伐他汀通过影响组织再生的不同方面来促进伤口愈合过程；然而，需要进一步研究以找出这种化学制剂的确切机制、优缺点。
