Sikder Md Asaduzzaman, Lee Hyun Jae, Ryu Jiho, Park Su Hyun, Kim Ju-Ock, Hong Jang-Hee, Seok Jeong Ho, Lee Choong Jae
Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
Tuberc Respir Dis (Seoul). 2014 Mar;76(3):120-6. doi: 10.4046/trd.2014.76.3.120. Epub 2014 Mar 29.
We investigated whether wogonin and apigenin significantly affect the epidermal growth factor receptor (EGFR) signaling pathway involved in MUC5AC mucin gene expression, and production from cultured airway epithelial cells; this was based on our previous report that apigenin and wogonin suppressed MUC5AC mucin gene expression and production from human airway epithelial cells.
Confluent NCI-H292 cells were pretreated with wogonin or apigenin for 15 minutes or 24 hours and then stimulated with epidermal growth factor (EGF) for 24 hours or the indicated periods.
We found that incubation of NCI-H292 cells with wogonin or apigenin inhibited the phosphorylation of EGFR. The downstream signals of EGFR such as phosphorylation of MEK1/2 and ERK1/2 were also inhibited by wogonin or apigenin.
The results suggest that wogonin and apigenin inhibits EGFR signaling pathway, which may explain how they inhibit MUC5AC mucin gene expression and production induced by EGF.
我们研究了汉黄芩素和芹菜素是否显著影响参与MUC5AC粘蛋白基因表达及培养的气道上皮细胞分泌的表皮生长因子受体（EGFR）信号通路；这是基于我们之前的报告，即芹菜素和汉黄芩素可抑制人气道上皮细胞中MUC5AC粘蛋白基因的表达及分泌。
将汇合的NCI-H292细胞用汉黄芩素或芹菜素预处理15分钟或24小时，然后用表皮生长因子（EGF）刺激24小时或指定时间。
我们发现用汉黄芩素或芹菜素处理NCI-H292细胞可抑制EGFR的磷酸化。EGFR的下游信号，如MEK1/2和ERK1/2的磷酸化也被汉黄芩素或芹菜素抑制。
结果表明汉黄芩素和芹菜素可抑制EGFR信号通路，这可能解释了它们如何抑制EGF诱导的MUC5AC粘蛋白基因表达及分泌。
