Sharma Pratibha, Noronha Santosh
Department of Bioscience and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
ISRN Microbiol. 2014 Mar 10;2014:578682. doi: 10.1155/2014/578682. eCollection 2014.
Acetoin is widely used as flavor agent and serves as a precursor for chemical synthesis. Here we focused on identifying the best physiological conditions (initial substrate concentrations, pH, temperature, and agitation) for enhanced acetoin accumulation by Bacillus subtilis 168. The optimal physiological conditions support maximum acetoin accumulation by minimizing byproduct (acetate and butanediol) synthesis and a maximum of 75% enhancement in acetoin yield could be achieved. Additionally, the effect of change in ALS (acetolactate synthase) and ALDC (acetolactate decarboxylase) activities was evaluated on acetoin accumulation. Increasing ALS and ALDC enzyme activities led to efficient utilization of pyruvate towards acetoin accumulation and about 80% enhancement in acetoin accumulation was observed.
乙偶姻被广泛用作调味剂，并作为化学合成的前体。在此，我们着重确定通过枯草芽孢杆菌168增强乙偶姻积累的最佳生理条件（初始底物浓度、pH值、温度和搅拌）。最佳生理条件通过将副产物（乙酸和丁二醇）的合成降至最低来支持乙偶姻的最大积累，并且乙偶姻产量最多可提高75%。此外，还评估了乙酰乳酸合酶（ALS）和乙酰乳酸脱羧酶（ALDC）活性变化对乙偶姻积累的影响。ALS和ALDC酶活性的增加导致丙酮酸向乙偶姻积累的有效利用，并且观察到乙偶姻积累提高了约80%。
