Weber M N, Mósena A C S, Simões S V D, Almeida L L, Pessoa C R M, Budaszewski R F, Silva T R, Ridpath J F, Riet-Correa F, Driemeier D, Canal C W
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Patos, Paraíba, Brazil.
Transbound Emerg Dis. 2016 Feb;63(1):92-100. doi: 10.1111/tbed.12223. Epub 2014 Apr 16.
The genus Pestivirus of the family Flaviviridae consists of four recognized species: Bovine viral diarrhoea virus 1 (BVDV-1), Bovine viral diarrhoea virus 2 (BVDV-2), Classical swine fever virus (CSFV) and Border disease virus (BDV). Recently, atypical pestiviruses ('HoBi'-like pestiviruses) were identified in batches of contaminated foetal calf serum and in naturally infected cattle with and without clinical symptoms. Here, we describe the first report of a mucosal disease-like clinical presentation (MD) associated with a 'HoBi'-like pestivirus occurring in a cattle herd. The outbreak was investigated using immunohistochemistry, antibody detection, viral isolation and RT-PCR. The sequence and phylogenetic analysis of 5'NCR, N(pro) and E2 regions of the RT-PCR positive samples showed that four different 'HoBi'-like strains were circulating in the herd. The main clinical signs and lesions were observed in the respiratory and digestive systems, but skin lesions and corneal opacity were also observed. MD characteristic lesions and a pestivirus with cytopathic biotype were detected in one calf. The present study is the first report of a MD like presentation associated with natural infection with 'HoBi'-like pestivirus. This report describes the clinical signs and provides a pathologic framework of an outbreak associated with at least two different 'HoBi'-like strains. Based on these observations, it appears that these atypical pestiviruses are most likely underdiagnosed in Brazilian cattle.
牛病毒性腹泻病毒1型（BVDV - 1）、牛病毒性腹泻病毒2型（BVDV - 2）、猪瘟病毒（CSFV）和边界病病毒（BDV）。最近，在一批受污染的胎牛血清以及有或无临床症状的自然感染牛中发现了非典型瘟病毒（“HoBi”样瘟病毒）。在此，我们报告首例与牛群中出现的“HoBi”样瘟病毒相关的黏膜病样临床表现（MD）。通过免疫组织化学、抗体检测、病毒分离和逆转录 - 聚合酶链反应（RT - PCR）对此次疫情进行了调查。对RT - PCR阳性样本的5'非编码区（5'NCR）、N(pro)和E2区域进行序列和系统发育分析，结果显示该牛群中有四种不同的“HoBi”样毒株在传播。主要临床症状和病变出现在呼吸系统和消化系统，但也观察到了皮肤病变和角膜混浊。在一头犊牛中检测到了MD特征性病变和具有细胞病变生物型的瘟病毒。本研究是首例与“HoBi”样瘟病毒自然感染相关的MD样表现的报告。本报告描述了临床症状，并提供了与至少两种不同“HoBi”样毒株相关疫情的病理框架。基于这些观察结果，看来这些非典型瘟病毒在巴西牛群中很可能未得到充分诊断。
