首次在埃及一些持续性感染奶牛群中检测到新兴的 HoBi 样瘟病毒（BVD-3）。
First detection of emerging HoBi-like Pestivirus (BVD-3) among some persistently infected dairy cattle herds in Egypt.
机构信息
Virology Research Department, Animal Health Research Institute, Agriculture Research Center (ARC), 12618, Dokki, Giza, Egypt.
Biotechnology Research Unit, Animal Reproduction Research Institute, ARC, Al Haram, Egypt.
出版信息
Trop Anim Health Prod. 2022 Oct 7;54(6):336. doi: 10.1007/s11250-022-03332-2.
Bovine viral diarrhoea virus (BVDV) is a serious veterinary health concern worldwide. We conducted this study to determine the prevalence of persistent infections (PI) and identify the current strain among some dairy cattle herds in Egypt. A total of 240 serum samples were collected from six Egyptian provinces. Between 2019 and 2020, samples were tested by Enzyme linked immunosorbent assay (ELISA) for detection of PI animals, and then molecular characterization was performed. Six calves were found PI with a prevalence of 2.5% (6/240). Using molecular characterization, HoBi-like Pestivirus (BVD-3) was successfully identified in Egypt for the first time. Based on the BVD-3 reference strains on Genbank, the detected strains had an identity ranging from 98.8 to 99.6%. Partial nucleotide sequence of the 5'UTR gene for six tested samples was submitted to Genbank with accessions: OM324396, OM324397, OM324398, OM324399, OM3243100, and OM3243101.
牛病毒性腹泻病毒（BVDV）是全球范围内严重的兽医健康问题。我们进行了这项研究，以确定埃及部分奶牛群中持续感染（PI）的流行情况，并确定当前的毒株。从埃及六个省共采集了 240 份血清样本。2019 年至 2020 年期间，采用酶联免疫吸附试验（ELISA）检测 PI 动物，然后进行分子特征分析。结果发现 6 头小牛为 PI，流行率为 2.5%（6/240）。首次在埃及成功鉴定出 HoBi 样瘟病毒（BVD-3）。根据 Genbank 上的 BVD-3 参考株，检测到的毒株的同源性在 98.8%至 99.6%之间。对 6 个测试样本的 5'UTR 基因的部分核苷酸序列进行了提交，获得的登录号为 OM324396、OM324397、OM324398、OM324399、OM3243100 和 OM3243101。
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