Sener Asli Gamze, Afsar Ilhan, Demirci Mustafa
Ataturk Training and Research Hospital Medical Microbiology Laboratory, Izmir Katip Celebi University, Yesilyurt, Izmir, Turkey.
APMIS. 2014 Dec;122(12):1167-70. doi: 10.1111/apm.12275. Epub 2014 Apr 16.
The presence of antinuclear antibodies (ANAs), directed against intracellular antigens, is a hallmark of systemic autoimmune rheumatic diseases. The indirect immunofluorescence (IIF) assay is among the most commonly used routine methods for ANA detection as the screening test. The objective of the study was to evaluate ANA patterns in a 4-year period retrospectively. All 19 996 serum samples that were sent to the Laboratory of Medical Microbiology of the tertiary Hospital by any hospital department between 1 January 2009 and 1 January 2013 with a request to test for ANA, anti-ENA or both were included in the study. Of these samples, 4375 (21.9%) were ANA-IIF-positive and 15621 (78.1%) were ANA-IIF-negative. The presented ANA-positive samples consisted of 2392 (54.67%) homogenous, 818 (18.70%) speckled, 396 (9.05%) centromere, 242 (5.53%) nucleolar, 213 (4.87%) nuclear dots, 178 (4.07%) cytoplasmic (except for actin and golgi), 24 (0.55%) actin, 9 (0.21%) golgi, 53 (1.21%) nuclear membrane and 50 (1.14%) mixed pattern. Totally 7800 samples were examined by LIA. Of these samples, 3440 were positive and 4307 were negative with IIF and LIA. In addition, 22 samples were detected as IIF-positive but LIA-negative, whereas the rest 31 samples were IIF-negative but LIA-positive. ANA patterns in 22 IIF-positive samples were homogenous (9), speckled (5), golgi (4), cytoplasmic (3) and nucleolar (1). SSA/Ro-52, SSB/La and Scl-70 positivity were detected in 31 IIF-negative/LIA-positive samples by LIA. The present study comes forward with its overall scope, which covers 4-year data obtained in tertiary hospital located in the western part of Turkey.
抗核抗体（ANA）针对细胞内抗原，其存在是系统性自身免疫性风湿疾病的一个标志。间接免疫荧光（IIF）检测是作为筛查试验最常用的ANA常规检测方法之一。本研究的目的是回顾性评估4年期间的ANA模式。2009年1月1日至2013年1月1日期间，由三级医院的任何科室送至医学微生物学实验室，要求检测ANA、抗可提取性核抗原（ENA）或两者的所有19996份血清样本均纳入本研究。在这些样本中，4375份（21.9%）ANA-IIF检测呈阳性，15621份（78.1%）ANA-IIF检测呈阴性。呈现的ANA阳性样本包括2392份（54.67%）均质型、818份（18.70%）斑点型、396份（9.05%）着丝点型、242份（5.53%）核仁型、213份（4.87%）核点型、178份（4.07%）胞质型（肌动蛋白和高尔基体除外）、24份（0.55%）肌动蛋白型、9份（0.21%）高尔基体型、53份（1.21%）核膜型和50份（1.14%）混合型。总共7800份样本通过免疫印迹法（LIA）检测。在这些样本中，3440份IIF和LIA检测呈阳性，4307份呈阴性。此外，22份样本检测为IIF阳性但LIA阴性，其余31份样本检测为IIF阴性但LIA阳性。22份IIF阳性样本的ANA模式为均质型（9份）、斑点型（5份）、高尔基体型（4份）、胞质型（3份）和核仁型（1份）。通过LIA在31份IIF阴性/LIA阳性样本中检测到抗干燥综合征A抗原（SSA）/Ro-52、抗干燥综合征B抗原（SSB）/La和抗拓扑异构酶I（Scl-70）阳性。本研究以其全面的范围提出，其涵盖了在土耳其西部的三级医院获得的4年数据。
