Gupta Prishni, Priya Ritu, Nanda Rachita, Patel Suprava, Mohapatra Eli
Department of Biochemistry, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India.
J Lab Physicians. 2020 Aug;12(2):115-120. doi: 10.1055/s-0040-1716454. Epub 2020 Sep 2.
The incidence of autoimmune disorders has seen a rise in India in recent times. The symptoms and signs of these conditions are caused by a systemic autoimmune response, essentially characterized by the expression of the antinuclear antibodies (ANA). Presence of ANA in serum of patient could be the prime evidence of an autoimmune disorder. This study aimed to determine the antibody patterns and assess the clinical significance of ANA in patients of a teaching tertiary care hospital of central India. This retrospective cross-sectional data analysis study retrieved 538 reports of individuals, who were prescribed the ANA test by indirect immunofluorescence assay over a period of 11 months, from the archives of the Department of Biochemistry. For continuous data, student -test was used while Chi-square and Fisher exact was conducted for categorical data. A value less than 0.05 were taken as significant. Out of the 538 patients investigated for ANA testing by indirect immunofluorescence assay, 33% were positive, among which 74% were female, and majority belonged to the young adult age group. The most common pattern identified was nuclear, subpattern nuclear speckled. A high-ANA pattern positivity could be related to a high-autoimmunity prevalence in this region, and also promotes its use as a tool of evidence of suspected autoimmune disorders.
近年来，印度自身免疫性疾病的发病率呈上升趋势。这些疾病的症状和体征是由全身性自身免疫反应引起的，其本质特征是抗核抗体（ANA）的表达。患者血清中ANA的存在可能是自身免疫性疾病的主要证据。 本研究旨在确定印度中部一家教学三级医院患者的抗体模式，并评估ANA的临床意义。 这项回顾性横断面数据分析研究从生物化学系档案中检索了538份个体报告，这些个体在11个月的时间里通过间接免疫荧光法进行了ANA检测。对于连续数据，使用学生t检验，对于分类数据进行卡方检验和Fisher精确检验。P值小于0.05被认为具有统计学意义。 在通过间接免疫荧光法进行ANA检测的538例患者中，33%呈阳性，其中74%为女性，大多数属于年轻成年年龄组。最常见的模式是核型，亚模式为核斑点型。 ANA高模式阳性可能与该地区自身免疫性疾病的高患病率有关，也促进了其作为疑似自身免疫性疾病证据工具的应用。
