Wang Bing, Sun Yan-Fei, Song Na, Wei Jin-Ping, Wang Xiao-Jie, Feng Hao, Yin Zhi-Yuan, Kang Zhen-Sheng
College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
Plant Physiol Biochem. 2014 Jul;80:90-6. doi: 10.1016/j.plaphy.2014.03.020. Epub 2014 Mar 31.
MicroRNAs (miRNAs) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. Cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. In this study, we identified 31 known miRNAs and 3 novel miRNAs in wheat. Moreover, 19 stress-regulated miRNAs using RT-qPCR data in which the effects of three stresses were surveyed from the known miRNAs. Among them, 16, 12 and 8 miRNAs were regulated under cold, wounding and high-salinity treatments, respectively. Of which 4 miRNAs were highly responsive to cold stress in wheat by northern blot, and 6 wounding-regulated and 3 high-salinity-regulated miRNAs were detected. Meanwhile, miR159, miR393 and miR398 were responsive to multiple stress stimuli. Besides, 2 novel miRNAs were regulated by cold stress. While, the analyses of targets suggested miR159, miR398 and miR6001 could responses to stress conditions in regulation pathways. Taken together, the results of this study suggest that wheat miRNAs may play important roles in response to abiotic stress.
微小RNA（miRNA）在转录后调控中发挥关键作用，并且作为重要的内源性调节因子应对各种胁迫。低温、创伤和高盐是影响作物生长发育的三种常见环境胁迫刺激。在本研究中，我们在小麦中鉴定出31个已知miRNA和3个新的miRNA。此外，利用RT-qPCR数据从已知miRNA中检测出19个受胁迫调控的miRNA，其中三种胁迫的影响已被研究。其中，分别有16、12和8个miRNA在低温、创伤和高盐处理下受到调控。通过Northern杂交在小麦中发现4个miRNA对低温胁迫高度响应，同时检测到6个受创伤调控和3个受高盐调控的miRNA。此外，miR159、miR393和miR398对多种胁迫刺激有响应。另外，2个新的miRNA受低温胁迫调控。同时，靶标分析表明miR159、miR398和miR6001可能在调控途径中对胁迫条件作出响应。综上所述，本研究结果表明小麦miRNA可能在应对非生物胁迫中发挥重要作用。
