Cortell Carmela, Salvetti Pascal, Joly Thierry, Viudes-de-Castro Maria Pilar
Centro de Investigación y Tecnología Animal (CITA).Instituto Valenciano de Investigaciones Agrarias (IVIA).Polígono La Esperanza nº100,12.400 Segorbe (Castellón),Spain.
UNCEIA,Department of Research and Development,13 rue Jouet,94704 Maisons-Alfort,France.
Zygote. 2015 Aug;23(4):507-13. doi: 10.1017/S0967199414000112. Epub 2014 Apr 15.
Ovarian stimulation protocols are used usually to increase the number of oocytes collected. The determination of how oocyte quality may be affected by these superovulation procedures, therefore, would be very useful. There is a high correlation between oocyte ATP concentration and developmental competence of the resulting embryo. The aim of this study was to evaluate the effect of follicle stimulating hormone (FSH) origin and administration protocols on oocyte ATP content. Rabbit does were distributed randomly into four groups: (i) a control group; (ii) the rhFSH3 group: females were injected, every 24 h over 3 days, with 0.6 μl of rhFSH diluted in polyvinylpyrrolidone (PVP); (iii) the pFSH3 group: females were injected every 24 h over 3 days with 11.4 μg of pFSH diluted in PVP; and (iv) the pFSH5 group: females were injected twice a day for 5 days with 11.4 μg of pFSH diluted in saline serum. Secondly, the effect of pFSH5 protocol on developmental potential was evaluated. Developmental competence of oocytes from the control and pFSH5 groups was examined. Differences in superovulation treatments were found for ATP levels. In the pFSH5 group, the ATP level was significantly lower than that of the other groups (5.63 ± 0.14 for pFSH group versus 6.42 ± 0.13 and 6.19 ± 0.15 for rhFSH3 and pFSH3, respectively; P < 0.05). In a second phase, only 24.28% of pFSH5 ova developed into hatched blastocysts compared with 80.39% for the control group. A negative effect on oocyte quality was observed in the pFSH5 group in ATP production, it is possible that, after this superovulation treatment, oocyte metabolism would be affected.
卵巢刺激方案通常用于增加采集的卵母细胞数量。因此，确定这些超排卵程序如何影响卵母细胞质量将非常有用。卵母细胞ATP浓度与所得胚胎的发育能力之间存在高度相关性。本研究的目的是评估促卵泡激素（FSH）来源和给药方案对卵母细胞ATP含量的影响。将兔分为四组：（i）对照组；（ii）重组人促卵泡激素3组（rhFSH3组）：雌性在3天内每24小时注射0.6μl稀释于聚乙烯吡咯烷酮（PVP）中的重组人促卵泡激素；（iii）垂体促卵泡激素3组（pFSH3组）：雌性在3天内每24小时注射11.4μg稀释于PVP中的垂体促卵泡激素；（iv）垂体促卵泡激素5组（pFSH5组）：雌性每天注射两次，共5天，每次注射11.4μg稀释于生理盐水血清中的垂体促卵泡激素。其次，评估pFSH5方案对发育潜力的影响。检查了对照组和pFSH5组卵母细胞的发育能力。发现超排卵处理在ATP水平上存在差异。在pFSH5组中，ATP水平显著低于其他组（pFSH组为5.63±0.14，而重组人促卵泡激素3组和垂体促卵泡激素3组分别为6.42±0.13和6.19±0.15；P<0.05）。在第二阶段，只有24.28%的pFSH5卵发育成孵化囊胚，而对照组为80.39%。在pFSH5组中观察到对卵母细胞质量在ATP产生方面有负面影响，有可能在这种超排卵处理后，卵母细胞代谢会受到影响。
