Walker R L, Greene R T, Nicholson W L, Levine J F
Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Vet Microbiol. 1989 Apr;19(4):361-71. doi: 10.1016/0378-1135(89)90101-6.
Antigenic cross-reactivity between Borrelia burgdorferi and Borrelia anserina was studied using mouse immune sera and monoclonal antibodies. With immune sera, significant cross-reactivity between B. burgdorferi and B. anserina was demonstrated by indirect immunofluorescent assay. In immunoblots, most of the cross-reactivity was shown to be associated with the periplasmic flagella. Using monoclonal antibodies in immunoblots, it was shown that B. burgdorferi and B. anserina shared at least two flagellar epitopes, one of which was not shared with Borrelia hermsii or Borrelia coriaceae. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of whole cell lysates and the use of a species-specific monoclonal antibody (H5332) which reacts with a major outer surface protein (Osp A) of B. burgdorferi readily differentiated the two species at the molecular level.
利用小鼠免疫血清和单克隆抗体研究了伯氏疏螺旋体和鹅疏螺旋体之间的抗原交叉反应性。采用免疫血清，通过间接免疫荧光试验证实了伯氏疏螺旋体和鹅疏螺旋体之间存在显著的交叉反应性。在免疫印迹中，大部分交叉反应性被证明与周质鞭毛有关。在免疫印迹中使用单克隆抗体表明，伯氏疏螺旋体和鹅疏螺旋体至少共有两个鞭毛表位，其中一个表位与赫氏疏螺旋体或科氏疏螺旋体不共有。全细胞裂解物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱以及使用与伯氏疏螺旋体主要外表面蛋白（Osp A）反应的种特异性单克隆抗体（H5332）在分子水平上很容易区分这两个菌种。
