Eiffert H, Lotter H, Jarecki-Khan K, Thomssen R
Department of Medical Microbiology, Georg-August-University, Göttingen, Federal Republic of Germany.
Med Microbiol Immunol. 1991;180(5):229-37. doi: 10.1007/BF00202557.
Investigations of immunoblots using Borrelia burgdorferi antigen demonstrated that a band, migrating faster than the bromophenol blue front in sodium dodecyl sulfate-gel electrophoresis, reacted strongly with sera containing anti-Borrelia burgdorferi antibodies preferentially of the IgG class. Extraction of this antigenic component and chemical analyses showed that the substance was composed mainly of fatty acids and carbohydrates. Typical structures of classical lipooolysaccharides such as 3-deoxy-D-manno-2-octulosonic acid, hydroxy fatty acids or lipid A could not be detected.
使用伯氏疏螺旋体抗原进行免疫印迹研究表明，在十二烷基硫酸钠凝胶电泳中，有一条带迁移速度比溴酚蓝前沿快，它与含有优先为IgG类抗伯氏疏螺旋体抗体的血清发生强烈反应。对该抗原成分进行提取和化学分析表明，该物质主要由脂肪酸和碳水化合物组成。未检测到典型的经典脂多糖结构，如3-脱氧-D-甘露-2-辛酮糖酸、羟基脂肪酸或脂质A。
