Yu Jingjing, Wang Sheng, Zhao Ge, Wang Bing, Ding Li, Zhang Xiaobing, Xie Jianping, Xie Fuwei
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 May 1;958:130-5. doi: 10.1016/j.jchromb.2014.03.023. Epub 2014 Mar 31.
Urinary aromatic amines (AAs) could be used as biomarkers for human exposure to AAs in cigarette smoke. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of urinary AAs (i.e. 1-naphthylamine (1-NA), 2-naphthylamine (2-NA), 3-aminobiphenyl (3-ABP) and 4-aminobiphenyl (4-ABP)) in smokers and nonsmokers. A molecularly imprinted polymers (MIPs) solid phase extraction (SPE) cartridge was applied to purify urine samples and no derivatization reaction was involved. Each analytes used respective stable isotope internal standards, which could well compensate matrix effect. Lower limit of detections (LODs) for four AAs were obtained and in the range of 1.5-5ngL(-1). Recovery ranged from 87.7±4.5% to 111.3±6.4% and precision were less than 9.9%. The method was applied to analyze urine samples of 40 smokers and 10 nonsmokers. The 24h urinary excretion amounts of total AAs were higher for smokers compared with nonsmokers. What's more, 1-NA, 3-ABP and 4-ABP excretion amounts showed significant differences (p<0.05) between smokers and nonsmokers.
尿中芳香胺（AAs）可作为人体接触香烟烟雾中AAs的生物标志物。建立了一种液相色谱-串联质谱（LC-MS/MS）法，用于测定吸烟者和非吸烟者尿中的AAs（即1-萘胺（1-NA）、2-萘胺（2-NA）、3-氨基联苯（3-ABP）和4-氨基联苯（4-ABP））。应用分子印迹聚合物（MIPs）固相萃取（SPE）柱对尿液样本进行净化，且不涉及衍生化反应。每种分析物使用各自的稳定同位素内标，可很好地补偿基质效应。获得了四种AAs的检测下限（LODs），范围为1.5-5ngL（-1）。回收率在87.7±4.5%至111.3±6.4%之间，精密度小于9.9%。该方法应用于分析40名吸烟者和10名非吸烟者的尿液样本。吸烟者的总AAs 24小时尿排泄量高于非吸烟者。此外，吸烟者和非吸烟者之间的1-NA、3-ABP和4-ABP排泄量存在显著差异（p<0.05）。
