Nolte H, Kruse A, Stahl Skov P, Schiøtz P O
Department of Pediatrics, University Hospital of Aarhus, Denmark.
Agents Actions. 1989 Apr;27(1-2):93-6. doi: 10.1007/BF02222208.
Dispersed human intestinal mast cells were used for passive sensitization experiments. Eight biopsies (9.7 +/- 1.2 mg/biopsy) of human duodenum were collected from non-atopic children (5) and adults (5). The tissue was dispersed mechanically and enzymatically to yield single cell suspensions. The method produced 2 x 10(3) mast cells per mg wet weight of tissue in a purity of 2.8%. Passive sensitization of the mast cells was performed with the patients' own plasma and plasma obtained from atopic donors. The non-atopic mast cells were able to bind the allergen-specific IgE. In addition, passive sensitization with atopic donor-plasma enhanced the cell sensitivity and cell reactivity to anti-IgE challenge, but had no effect on the cellular response to the ionophore A23187. The study shows that the enzymatic dispersion of human intestinal mast cells produces functionally intact mast cells with preserved Fc-receptors which can be passively sensitized.
分散的人肠道肥大细胞用于被动致敏实验。从非特应性儿童（5名）和成人（5名）中收集8份人十二指肠活检组织（每份活检组织9.7±1.2毫克）。将组织进行机械和酶处理以产生单细胞悬液。该方法每毫克湿重组织产生2×10³个肥大细胞，纯度为2.8%。用患者自身血浆和来自特应性供体的血浆对肥大细胞进行被动致敏。非特应性肥大细胞能够结合变应原特异性IgE。此外，用特应性供体血浆进行被动致敏增强了细胞对抗IgE激发的敏感性和细胞反应性，但对细胞对离子载体A23187的反应没有影响。该研究表明，人肠道肥大细胞的酶分散产生功能完整且保留Fc受体的肥大细胞，这些肥大细胞可被被动致敏。
