Skov P S, Permin H, Malling H J
Scand J Immunol. 1977;6(10):1021-8. doi: 10.1111/j.1365-3083.1977.tb00338.x.
IgE was removed from human basophils of 4 nonatopic persons and 10 hay fever patients allergic to timothy grass pollen by treating the cells with buffer adjusted to pH 4. IgE could be removed and refixed to the same cells. Refixation was demonstrated by immunofluorescence and by the ability of basophils to release histamine on exposure to timothy pollen. Removed total IgE and specific IgE directed against timothy pollen were estimated, and a linear correlation to the level of total IgE and specific IgE in serum was found. The total number of IgE molecules per basophil was calculated to be in the range of 30,000 to 300,000, and timothy-specific IgE constituted 4%-15% of the total IgE molecules on the cells. It was furthermore established that specific cell-bound IgE was linearly correlated to the pollen concentration releasing 20% of the histamine contents of the basophils. Separated 2gE from sensitized and nonsensitized basophils could be bound to basophils from other patients, resulting in a change in cell sensitivity. This could be ascribed to additional binding to free cell receptors as well as to a partial replacement of bound IgE. Basophils from non-atopic persons could not be sensitized by incubation with surface IgE from atopic persons. The results indicate that acid treatment is a simple method suitable for removing IgE from basophils. This IgE is intact and can be quantitated.
通过用pH值调至4的缓冲液处理细胞，从4名非特应性个体和10名对梯牧草花粉过敏的花粉症患者的人嗜碱性粒细胞中去除IgE。IgE可以被去除并重新固定到相同的细胞上。通过免疫荧光以及嗜碱性粒细胞在接触梯牧草花粉时释放组胺的能力证明了重新固定。对去除的总IgE和针对梯牧草花粉的特异性IgE进行了估计，并发现其与血清中总IgE和特异性IgE水平呈线性相关。计算得出每个嗜碱性粒细胞的IgE分子总数在30,000至300,000之间，且针对梯牧草的特异性IgE占细胞上总IgE分子的4%-15%。此外，还确定特异性细胞结合IgE与释放嗜碱性粒细胞20%组胺含量的花粉浓度呈线性相关。从致敏和未致敏嗜碱性粒细胞中分离出的IgE可以与其他患者的嗜碱性粒细胞结合，导致细胞敏感性发生变化。这可能归因于与游离细胞受体的额外结合以及结合IgE的部分替代。非特应性个体的嗜碱性粒细胞不能通过与特应性个体的表面IgE孵育而致敏。结果表明，酸处理是一种适合从嗜碱性粒细胞中去除IgE的简单方法。这种IgE是完整的，可以进行定量。
