Rojko N, Cronin B, Danial J S H, Baker M A B, Anderluh G, Wallace M I
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Department of Chemistry, Oxford University, Oxford, UK.
Biophys J. 2014 Apr 15;106(8):1630-7. doi: 10.1016/j.bpj.2013.11.4507.
Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin, Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ionic flux through individual pores in vitro, we observe that EqtII pores form predominantly within the liquid-disordered phase. We observe preferential binding of labeled EqtII at liquid-ordered/liquid-disordered domain boundaries before it accumulates in the liquid-disordered phase.
利用相分离的液滴界面双层膜，我们观察到一种真核细胞溶素——刺尾鱼毒素II（EqtII）的膜结合和孔形成。已知EqtII的活性取决于靶膜中鞘磷脂的存在，并且脂质相分离会增强其活性。通过体外成像单个孔中的离子通量，我们观察到EqtII孔主要在液相无序相中形成。我们观察到标记的EqtII在积累到液相无序相之前优先结合在液相有序/液相无序域边界处。
