Sopić M, Bogavac-Stanojević N, Baralić I, Kotur-Stevuljević J, Đorđević B, Stefanović A, Jelić-Ivanović Z
Department of Medical Biochemistry Faculty of Pharmacy, University of Belgrade Belgrade, Serbia -
J Sports Med Phys Fitness. 2014 Jun;54(3):354-61.
The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent.
This study was conducted on 16 soccer players aged 18.13±0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups).
During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and PAB (P=0.045) were significantly lower after training. After 45 days training period DNA score was decreased but with no significance. However, percent of cells with medium and high damage was significantly lower (P=0.01). TOS (P=0.001) and MDA (P=0.038) levels were also significantly lower, while sulphydryl-groups levels were significantly higher (P=0.006).
This study demonstrated that single soccer training had not compromised DNA stability. Possible development of oxidative stress was effectively neutralized by very well preserved antioxidative mechanisms. It was also shown that during 45 days adaptive response was induced. All measured parameters should be considered as useful information on oxidative status of trainees.
本研究旨在确定单次足球训练是否会导致氧化应激和DNA不稳定。我们还试图研究在45天的训练期内是否会产生适应性反应以及其程度如何。
本研究对16名年龄为18.13±0.35岁的足球运动员进行。我们使用单细胞凝胶电泳（彗星试验）来研究白细胞DNA稳定性。结果以DNA评分以及中度和高度损伤细胞的百分比表示。通过血液中超氧阴离子、硫代巴比妥酸反应物质（TBARs）、总抗氧化状态（TAS）、总氧化状态（TOS）、促氧化剂-抗氧化剂平衡（PAB）和巯基（SH-基团）水平来评估受试者的氧化状态。
在单次足球训练期间，训练后DNA评分以及中度和高度损伤细胞的百分比有所增加，但无统计学意义。训练后巯基（P=0.033）、TOS（P=0.002）和PAB（P=0.045）显著降低。在45天的训练期后，DNA评分下降但无统计学意义。然而，中度和高度损伤细胞的百分比显著降低（P=0.01）。TOS（P=0.001）和丙二醛（MDA）（P=0.038）水平也显著降低，而巯基水平显著升高（P=0.006）。
本研究表明单次足球训练并未损害DNA稳定性。保存良好的抗氧化机制有效中和了可能产生的氧化应激。研究还表明在45天内诱导了适应性反应。所有测量参数都应被视为有关受训者氧化状态的有用信息。
