Daniels C J, Hofman J D, MacWilliam J G, Doolittle W F, Woese C R, Luehrsen K R, Fox G E
Mol Gen Genet. 1985;198(2):270-4. doi: 10.1007/BF00383005.
We show that the archaebacterium Halobacterium volcanii contains two ribosomal RNA gene clusters, in which genes for individual rRNAs lie in the order 16S-23S-5S. We have cloned the 5S rRNA genes of both clusters and present sequences of the two 5S rRNA genes and their 5' and 3' flanking regions, as well as the sequence of H. volcanii 5S rRNA. We show that a gene for a tRNACys lies downstream from one, but not the other, 5S rRNA gene, and have obtained evidence that this tRNA gene is transcribed in vivo. We discuss regions of potential secondary structure which may be involved in transcription termination. We note regions of unexpected flanking sequence conversation both within H. volcanii 5S rRNA gene regions, and between them and the corresponding 5S rRNA gene region of H. cutirubrum (Hui and Dennis 1984).
我们发现嗜盐古菌嗜盐栖热菌含有两个核糖体RNA基因簇，其中各个rRNA的基因排列顺序为16S - 23S - 5S。我们克隆了两个基因簇的5S rRNA基因，并给出了两个5S rRNA基因及其5'和3'侧翼区域的序列，以及嗜盐栖热菌5S rRNA的序列。我们发现一个tRNACys基因位于其中一个而非另一个5S rRNA基因的下游，并且有证据表明该tRNA基因在体内会被转录。我们讨论了可能参与转录终止的潜在二级结构区域。我们注意到在嗜盐栖热菌5S rRNA基因区域内，以及它们与嗜盐栖红菌（Hui和Dennis，1984）相应的5S rRNA基因区域之间，存在意外的侧翼序列保守区域。
