Félix Nuno M, Leal Rodolfo O, Goy-Thollot I, Walton Ronald S, Gil Solange A, Mateus Luísa M, Matos Ana S, Niza Maria M R E
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA) Faculdade de Medicina Veterinária, University of Lisbon (ULisboa), Lisboa, Portugal.
Centre Hospitalier Vétérinaire Fregis, Arcueil, France.
Iran J Basic Med Sci. 2017 Apr;20(4):368-379. doi: 10.22038/IJBMS.2017.8576.
Buprenorphine is a common analgesic in experimental research, due to effectiveness and having few side-effects, including a limited influence in the immune and endocrine systems. However, how buprenorphine affects cytokine levels and the adrenal and thyroid response during general anesthesia and surgery is incompletely understood. This study aimed to assess whether buprenorphine modulated significantly those responses in rats submitted to general anesthesia, mechanical ventilation, and surgical insertion of intravascular catheters.
Animals were anesthetized with isoflurane, mechanically ventilated, and surgically instrumented for carotid artery and the femoral vein catheter placement. The test group (n=16), received buprenorphine subcutaneously before surgery, whereas the control group (n=16) received normal saline. Blood sampling to determine plasma levels of adrenocorticotropic hormone (ACTH), corticosterone (CS), total thyroxine (TT4), total triiodothyronine (TT3), thyroid-stimulating hormone (TSH), TNF-α, IL6, IL10, TNF-α, IL6, and IL10 mRNA was performed at 10 min after completion of all surgical procedures and at 90, 150, 240, and 300 min thereafter, with the animals still anesthetized and with mechanical ventilation.
Buprenorphine-treated animals had higher levels of ACTH, CS, and TT4 at several time points (<0.05) and TSH and TT3 at all-time points (<0.05). They also had increased IL10, -α, and mRNA levels.
In this model, buprenorphine significantly modulated the intra-operative cytokine and endocrine response to anesthesia, mechanical ventilation, and surgical placement of intravascular catheters. The mechanism and significance of these findings remain undetermined. Researchers should be aware of these effects when considering the use of buprenorphine for analgesic purposes.
丁丙诺啡是实验研究中常用的镇痛药，因其有效性和副作用少，包括对免疫和内分泌系统影响有限。然而，丁丙诺啡在全身麻醉和手术期间如何影响细胞因子水平以及肾上腺和甲状腺反应尚不完全清楚。本研究旨在评估丁丙诺啡是否能显著调节接受全身麻醉、机械通气和血管内导管手术插入的大鼠的这些反应。
动物用异氟烷麻醉，机械通气，并通过手术进行颈动脉和股静脉导管置入。试验组（n = 16）在手术前皮下注射丁丙诺啡，而对照组（n = 16）接受生理盐水。在所有手术操作完成后10分钟以及此后90、150、240和300分钟采集血样，以测定血浆促肾上腺皮质激素（ACTH）、皮质酮（CS）、总甲状腺素（TT4）、总三碘甲状腺原氨酸（TT3）、促甲状腺激素（TSH）、TNF-α、IL6、IL10、TNF-α、IL6和IL10 mRNA水平，此时动物仍处于麻醉状态并进行机械通气。
丁丙诺啡治疗的动物在几个时间点的ACTH、CS和TT4水平较高（<0.05），TSH和TT3在所有时间点均较高（<0.05）。它们的IL10、-α和mRNA水平也有所升高。
在该模型中，丁丙诺啡显著调节了术中对麻醉、机械通气和血管内导管手术置入的细胞因子和内分泌反应。这些发现的机制和意义尚不确定。研究人员在考虑使用丁丙诺啡进行镇痛时应注意这些影响。
