短寡核苷酸对DNA水解脱氧核酶的变构调控及其在DNA逻辑门中的应用。

Allosteric control of a DNA-hydrolyzing deoxyribozyme with short oligonucleotides and its application in DNA logic gates.

作者信息

Furukawa Kazuhiro, Minakawa Noriaki

机构信息

Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1-78-1, Tokushima 770-8505, Japan.

出版信息

Org Biomol Chem. 2014 Jun 7;12(21):3344-8. doi: 10.1039/c4ob00451e. Epub 2014 Apr 17.

DOI:10.1039/c4ob00451e

PMID:24740418

Abstract

Allosteric control of deoxyribozymes is useful for a broad range of practical applications, such as nucleic acid sensing and DNA-computing. We found that the catalytic activity of a DNA-hydrolyzing deoxyribozyme could be allosterically regulated by adding short oligonucleotides. We used this technique to construct deoxyribozyme-based logic gates.

摘要

脱氧核酶的变构控制对于广泛的实际应用很有用，例如核酸传感和DNA计算。我们发现，通过添加短寡核苷酸可以变构调节一种DNA水解脱氧核酶的催化活性。我们利用这项技术构建了基于脱氧核酶的逻辑门。