Stojanovic Milan N, Mitchell Tiffany Elizabeth, Stefanovic Darko
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York 10032, USA.
J Am Chem Soc. 2002 Apr 10;124(14):3555-61. doi: 10.1021/ja016756v.
We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open the possibility of communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.
我们在此报告一组基于脱氧核酶的逻辑门，它们能够生成任何布尔函数。我们构建了基本的非门和与门，随后是更复杂的异或门。这些门是通过将分子信标茎环与锤头型脱氧核酶相结合的模块化设计构建而成。重要的是，由于这些门的输入和输出均为寡核苷酸，它们开启了溶液中各种计算元件之间进行通信的可能性。这些门的操作可方便地连接到荧光读数。
