在幽门螺杆菌疫苗接种小鼠模型中，呈现白细胞介素-2的枯草芽孢杆菌孢子的黏膜佐剂活性。
Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination.
作者信息
Hinc Krzysztof, Stasiłojć Małgorzata, Piątek Iwona, Peszyńska-Sularz Grażyna, Isticato Rachele, Ricca Ezio, Obuchowski Michał, Iwanicki Adam
机构信息
Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
出版信息
PLoS One. 2014 Apr 17;9(4):e95187. doi: 10.1371/journal.pone.0095187. eCollection 2014.
Abstract
The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.
摘要
枯草芽孢杆菌的内生孢子目前被广泛用作展示异源蛋白的平台，由于其安全记录以及对恶劣环境条件的高抗性，可被视为口服疫苗的潜在载体。在本研究中，我们表明，表达牙龈弯曲杆菌尿素酶B（UreB）蛋白片段并在营养启动子下表达ureB基因的重组枯草芽孢杆菌孢子，与表达白细胞介素-2（IL-2）的孢子共同给药时，在口服免疫的小鼠中引发了强烈的细胞免疫反应。我们首次展示了两种重组孢子，一种携带抗原，另一种携带佐剂，在单次口服免疫中的成功应用。
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