Bakonyi Tamás, Busquets Núria, Nowotny Norbert
1 Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna , Vienna, Austria .
Vector Borne Zoonotic Dis. 2014 May;14(5):324-9. doi: 10.1089/vbz.2013.1510. Epub 2014 Apr 18.
The complete genomic sequence of Usutu virus (USUV, genus Flavivirus, family Flaviviridae) strain MB119/06, detected in a pool of Culex pipiens mosquitoes in northeastern Spain (Viladecans, Catalonia) in 2006, was determined and analyzed. The phylogenetic relationship with all other available complete USUV genome sequences was established. The Spanish sequence investigated showed the closest relationship to the USUV prototype strain SA AR 1776 isolated in South Africa in 1959 (96.9% nucleotide and 98.8% amino acid identities). Conserved structural elements and enzyme motifs of the putative polyprotein precursor were identified. Unique amino acid substitutions were recognized; however, their potential roles as virulence markers could not be verified. Comparisons of the polyprotein precursor sequences of USUV strains detected in mosquitoes, birds, and humans could not confirm the predicted role of unique amino acid substitutions in relation to virulence in humans. Phylogenetic analysis of a partial coding section of the NS5 protein gene region indicated that USUV strains circulating in Europe form three different genetic clusters. Broad and targeted surveys for USUV in mosquitoes could reveal further details of the geographic distribution and genetic diversity of the virus in Europe and in Africa.
2006年在西班牙东北部（加泰罗尼亚自治区比拉代坎斯）的一批尖音库蚊中检测到乌苏图病毒（USUV，黄病毒属，黄病毒科）MB119/06株，并对其完整基因组序列进行了测定和分析。确定了该病毒与所有其他可用的完整乌苏图病毒基因组序列之间的系统发育关系。所研究的西班牙序列与1959年在南非分离出的乌苏图病毒原型株SA AR 1776关系最为密切（核苷酸同一性为96.9%，氨基酸同一性为98.8%）。鉴定了假定多蛋白前体的保守结构元件和酶基序。识别出了独特的氨基酸替换；然而，无法证实它们作为毒力标志物的潜在作用。对在蚊子、鸟类和人类中检测到的乌苏图病毒株多蛋白前体序列的比较，无法证实独特氨基酸替换与人类毒力相关的预测作用。对NS5蛋白基因区域部分编码区的系统发育分析表明，在欧洲传播的乌苏图病毒株形成了三个不同的基因簇。对蚊子中的乌苏图病毒进行广泛和有针对性的调查，可能会揭示该病毒在欧洲和非洲的地理分布和遗传多样性的更多细节。
