灭活全病毒在小鼠和非人灵长类动物中诱导针对甲型流感病毒H7N9的中和抗体
Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates.
作者信息
Pan Weiqi, Han Ling, Dong Zhenyuan, Niu Xuefeng, Li Zhengfeng, Bao Linlin, Li Chufang, Luo Qinfang, Yang Zifeng, Li Xiaobo, Huang Jicheng, Feng Liqiang, Qin Chuan, Zhong Nanshan, Chen Ling
机构信息
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Anhui University, Anhui, China.
出版信息
Antiviral Res. 2014 Jul;107:1-5. doi: 10.1016/j.antiviral.2014.04.003. Epub 2014 Apr 16.
We evaluated the immunogenicity of hemagglutinin (HA) in the context of inactivated H7N9/AH/1/13-PR8 whole-virion. At 4weeks after immunization with 15μg HA, mice produced hemagglutination inhibition (HI) titers of 1:192 and neutralizing antibodies of 1:317. Aluminum hydroxide (alum), or a booster immunization, or both increased HI to 1:768, 1:384, 1:896 and neutralizing antibodies to 1:1868, 1:2302, 1:10,000, respectively. Macaques generated HI of 1:190 or 1:360 and virus neutralizing titers of 1:280 or 1:658 at 3weeks after immunization with HA alone or with alum. Sera from immunized mice and macaques protected mice from infection of A/Anhui/1/2013 (H7N9), suggesting an H7N9 vaccine is immunologically feasible.
我们在灭活的H7N9/AH/1/13-PR8全病毒背景下评估了血凝素（HA）的免疫原性。用15μg HA免疫4周后，小鼠产生了1:192的血凝抑制（HI）效价和1:317的中和抗体。氢氧化铝（明矾）、加强免疫或两者同时使用可分别将HI效价提高到1:768、1:384、1:896，中和抗体提高到1:1868、1:2302、1:10,000。单独用HA或与明矾一起免疫3周后，猕猴产生了1:190或1:360的HI效价和1:280或1:658的病毒中和效价。免疫小鼠和猕猴的血清可保护小鼠免受A/安徽/1/2013（H7N9）感染，表明H7N9疫苗在免疫方面是可行的。
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