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一种用于通过快速筛选许可细胞进行病毒分离的微流控细胞芯片。

A microfluidic cell chip for virus isolation via rapid screening for permissive cells.

机构信息

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou, 450001, China.

出版信息

Virol Sin. 2022 Aug;37(4):547-557. doi: 10.1016/j.virs.2022.04.011. Epub 2022 May 2.

DOI:10.1016/j.virs.2022.04.011
PMID:35504535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9437619/
Abstract

Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way.

摘要

病毒鉴定不仅是病毒性传染病早期诊断的前提，也是有效预防疫情的前提。根据科赫假设，成功培养是鉴定病毒的金标准。然而，这需要筛选出允许的细胞系，这在传统上是耗时、试剂和劳动密集型的。在这里，报道了一种简单易用的微流控芯片，该芯片通过播种多种细胞系并平行培养而成，用于病毒培养和病毒允许的宿主细胞筛选。该芯片通过感染两种已知病毒，肠道病毒 71（EV71）和甲型 H1N1 流感病毒进行了测试。EV71 和 H1N1 的感染分别在 RD 和 MDCK 细胞中引起明显的细胞病变效应（CPE），表明基于这种微流控细胞芯片的病毒培养可以替代传统的平板培养方法，并再现病毒感染引起的典型 CPE。使用这种微流控细胞芯片方法进行病毒培养，可以实现对新出现病毒的高通量、自动化和前所未有的快速鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/b2287183610f/figs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/3b4016aed428/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/5efd2e836b83/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/902b38b69fe5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/2f1c32acdf46/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/dd41c84c92b2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/0fe6444c1068/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/f120383038b6/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/ef8a1ee60f59/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/8eabf478450f/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/1c0717e49262/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/f1ef4a675d48/figs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/094041fcaa54/figs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/b2287183610f/figs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/3b4016aed428/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/5efd2e836b83/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/902b38b69fe5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/2f1c32acdf46/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/dd41c84c92b2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/0fe6444c1068/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/f120383038b6/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/ef8a1ee60f59/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/8eabf478450f/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/1c0717e49262/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/f1ef4a675d48/figs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/094041fcaa54/figs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec0/9437619/b2287183610f/figs5.jpg

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