一种保守的 RNA 聚合酶 III 启动子，对于 γ疱疹病毒 TMER 转录和 microRNA 加工是必需的。
A conserved RNA polymerase III promoter required for gammaherpesvirus TMER transcription and microRNA processing.
机构信息
Program in Molecular Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA; Department of Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Department of Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
出版信息
Gene. 2014 Jul 1;544(1):8-18. doi: 10.1016/j.gene.2014.04.026. Epub 2014 Apr 16.
Abstract
Canonical RNA polymerase III (pol III) type 2 promoters contain a single A and B box and are well documented for their role in tRNA and SINE transcription in eukaryotic cells. The genome of Murid herpesvirus 4 (MuHV-4) contains eight polycistronic tRNA-microRNA encoded RNA (TMER) genes that are transcribed from a RNA pol III type 2-like promoter containing triplicated A box elements. Here, we demonstrate that the triplicated A box sequences are required in their entirety to produce functional MuHV-4 miRNAs. We also identify that these RNA pol III type 2-like promoters are conserved in eukaryotic genomes. Human and mouse predicted tRNA genes containing these promoters also show enrichment of alternative RNA pol III transcription termination sequences and are predicted to give rise to longer tRNA primary transcripts.
摘要
经典 RNA 聚合酶 III（pol III）类型 2 启动子包含单个 A 盒和 B 盒，并且在真核细胞中 tRNA 和 SINE 转录方面的作用已得到充分证明。Murid 疱疹病毒 4（MuHV-4）的基因组包含八个多顺反子 tRNA-微小 RNA 编码 RNA（TMER）基因，这些基因由包含重复 A 盒元件的 RNA pol III 类型 2 样启动子转录。在这里，我们证明了完整的三重复 A 盒序列对于产生功能性 MuHV-4 miRNA 是必需的。我们还发现这些 RNA pol III 类型 2 样启动子在真核基因组中是保守的。含有这些启动子的人类和小鼠预测 tRNA 基因也显示出替代 RNA pol III 转录终止序列的富集，并且预计会产生更长的 tRNA 初级转录物。
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