Aydin Suna, Aydin Suleyman, Kobat Mehmet Ali, Kalayci Mehmet, Eren Mehmet Nesimi, Yilmaz Musa, Kuloglu Tuncay, Gul Evrim, Secen Ozlem, Alatas Omer Dogan, Baydas Adil
Department of Cardiovascular Surgery, Elazig Research and Education Hospital, Elazig 23100, Turkey; Firat University, School of Medicine, Department of Anatomy, Elazig 23119, Turkey.
Firat University, School of Medicine, Department of Medical Biochemistry (Firat Hormones Research Group), Elazig 23119, Turkey.
Peptides. 2014 Jun;56:141-5. doi: 10.1016/j.peptides.2014.04.002. Epub 2014 Apr 18.
Irisin is a muscle-secreted protein. Cardiac muscle produces more irisin than skeletal muscle in response to acute exercise, and is associated with myocardial infarction (MI) in an experimental model induced by isoproterenol in rats. The timing and significance of its release in patients with acute myocardial infarction (AMI) needs further investigation. We have studied the relationship between serum/saliva irisin concentration and AMI in humans. Serum and saliva samples were taken within 3 days of admission in 11 patients with AMI and in 14 matched controls. Salivary gland irisin was detected immunohistochemically, and serum and saliva levels were measured by ELISA. The three major paired salivary glands (submandibular, sublingual and parotid) produce and release irisin into saliva. Troponin-I, CK, CK-MB concentrations in the AMI group gradually increased from up to 12h, while saliva and serum irisin gradually decreased from up to 48 h, compared with the control group (P<0.05). After 12h, troponin-I, CK, CK-MB started to decrease, while saliva and serum irisin started to increase at 72 h. Serum irisin levels correlated with age, while troponin I, CK-MB, and CK were correlated and with saliva irisin in AMI patients. Besides cardiac troponin and CK-MB, irisin adds new diagnostic information in AMI patients, and the gradual decrease of saliva/serum irisin over 48 h could be a useful biomarker.
鸢尾素是一种由肌肉分泌的蛋白质。在急性运动后，心肌产生的鸢尾素比骨骼肌更多，并且在大鼠异丙肾上腺素诱导的实验模型中，鸢尾素与心肌梗死（MI）相关。其在急性心肌梗死（AMI）患者中释放的时间和意义需要进一步研究。我们研究了人类血清/唾液鸢尾素浓度与AMI之间的关系。在11例AMI患者和14例匹配的对照者入院3天内采集血清和唾液样本。通过免疫组织化学检测唾液腺鸢尾素，并采用酶联免疫吸附测定法测量血清和唾液水平。三大对唾液腺（下颌下腺、舌下腺和腮腺）产生鸢尾素并将其释放到唾液中。与对照组相比，AMI组肌钙蛋白-I、肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）浓度从最多12小时起逐渐升高，而唾液和血清鸢尾素从最多48小时起逐渐降低（P<0.05）。12小时后，肌钙蛋白-I、CK、CK-MB开始下降，而唾液和血清鸢尾素在72小时开始升高。AMI患者血清鸢尾素水平与年龄相关，而肌钙蛋白I、CK-MB和CK与唾液鸢尾素相关。除了心肌肌钙蛋白和CK-MB外，鸢尾素为AMI患者增添了新的诊断信息，并且唾液/血清鸢尾素在48小时内逐渐降低可能是一种有用的生物标志物。
