Wei Quanwei, Li Jian, Li Xingmei, Zhang Lei, Shi Fangxiong
Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Reprod Toxicol. 2014 Jul;46:121-8. doi: 10.1016/j.reprotox.2014.03.007. Epub 2014 Apr 18.
We investigated the reproductive toxicity of acrylamide in female mice. The results from immunohistochemistry provided evidence that nitric oxide synthase (NOS) signaling was involved in the process of follicular development and atresia. Oral administration of acrylamide to female mice led to significantly reduced body weights, organ weights and the number of corpora lutea (P<0.05). Serum progesterone concentrations were significantly reduced (P<0.05) concomitant with the increasing doses of acrylamide; however, 17β-estradiol (E2) concentrations were unchanged with treatment. Measurement of NOS activities indicated that total NOS (TNOS), iNOS and eNOS activities were significantly increased (P<0.05) with increasing doses of acrylamide. The results from in vitro study indicated that acrylamide reduced the viability of mouse granulosa cells in a dose-dependent manner. In summary, acrylamide affected bodily growth and development, as well as reproductive organs, the number of corpora lutea and progesterone production in female mice, possibly acting through the NOS signaling pathway.
我们研究了丙烯酰胺对雌性小鼠的生殖毒性。免疫组织化学结果提供了证据，表明一氧化氮合酶（NOS）信号通路参与卵泡发育和闭锁过程。给雌性小鼠口服丙烯酰胺导致体重、器官重量和黄体数量显著降低（P<0.05）。血清孕酮浓度随着丙烯酰胺剂量的增加而显著降低（P<0.05）；然而，17β-雌二醇（E2）浓度在处理后未发生变化。NOS活性测定表明，随着丙烯酰胺剂量的增加，总NOS（TNOS）、诱导型NOS（iNOS）和内皮型NOS（eNOS）活性显著增加（P<0.05）。体外研究结果表明，丙烯酰胺以剂量依赖的方式降低小鼠颗粒细胞的活力。总之，丙烯酰胺影响雌性小鼠的身体生长发育、生殖器官、黄体数量和孕酮产生，可能通过NOS信号通路发挥作用。
