Hirano T, Yasukawa K, Harada H, Taga T, Watanabe Y, Matsuda T, Kashiwamura S, Nakajima K, Koyama K, Iwamatsu A
Nature. 1986;324(6092):73-6. doi: 10.1038/324073a0.
When stimulated with antigen, B cells are influenced by T cells to proliferate and differentiate into antibody-forming cells. Since it was reported that soluble factors could replace certain functions of helper T cells in the antibody response, several different kinds of lymphokines and monokines have been reported in B-cell growth and differentiation. Among these, human B-cell differentiation factor (BCDF or BSF-2) has been shown to induce the final maturation of B cells into immunoglobulin-secreting cells. BSF-2 was purified to homogeneity and its partial NH2-terminal amino-acid sequence was determined. These studies indicated that BSF-2 is functionally and structurally unlike other known proteins. Here, we report the molecular cloning, structural analysis and functional expression of the cDNA encoding human BSF-2. The primary sequence of BSF-2 deduced from the cDNA reveals that BSF-2 is a novel interleukin consisting of 184 amino acids.
受到抗原刺激时，B细胞会受T细胞影响而增殖并分化为抗体形成细胞。自从有报道称可溶性因子可在抗体应答中替代辅助性T细胞的某些功能以来，已报道了几种不同的淋巴因子和单核因子参与B细胞的生长和分化。其中，人B细胞分化因子（BCDF或BSF-2）已被证明可诱导B细胞最终成熟为分泌免疫球蛋白的细胞。BSF-2已被纯化至同质，并确定了其部分NH2末端氨基酸序列。这些研究表明，BSF-2在功能和结构上与其他已知蛋白质不同。在此，我们报告编码人BSF-2的cDNA的分子克隆、结构分析和功能表达。从cDNA推导的BSF-2一级序列表明，BSF-2是一种由184个氨基酸组成的新型白细胞介素。
