Zheng Lan, Hao Long, Ma Hua, Tian Chengye, Li Tong, Sun Xinyi, Jia Mengshi, Jia Le
College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
Curr Microbiol. 2014 Sep;69(3):270-6. doi: 10.1007/s00284-014-0582-z. Epub 2014 Apr 20.
Cordyceps sinensis, a traditionally edible and medicinal fungus in China, cannot be artificially solid-cultured. Zinc (Zn), germanium (Ge), and selenium (Se) are the essential trace elements for human body. In this work, C. sinensis SU-01 was cultivated in liquid medium simultaneously containing Zn, Ge, and Se. The bioactive ingredients and in vivo antioxidant activities of Zn, Ge, Se-enriched mycelia (ZGSM) of C. sinensis SU-01 were investigated. Under the determined conditions, the Zn, Ge, and Se contents of ZGSM were 2543.16 ± 158.92, 1873.85 ± 81.82, and 1260.16 ± 107.12 μg/g, respectively. The optimal concentrations of Zn, Ge, and Se had a positive effect on biosynthesis of protein, polysaccharide, cordycepic acid, and amino acids. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) of mice blood were 3.72 ± 0.15 and 28.74 ± 2.53 % higher than that of control, respectively, and the content of malondialdehyde (MDA) was 41.01 ± 3.66 % lower than that of control.
冬虫夏草是中国一种传统的药食两用真菌，无法进行人工固体培养。锌（Zn）、锗（Ge）和硒（Se）是人体必需的微量元素。在本研究中，冬虫夏草SU - 01在同时含有Zn、Ge和Se的液体培养基中培养。对富锌、锗、硒的冬虫夏草SU - 01菌丝体（ZGSM）的生物活性成分及体内抗氧化活性进行了研究。在确定的条件下，ZGSM中Zn、Ge和Se的含量分别为2543.16±158.92、1873.85±81.82和1260.16±107.12μg/g。Zn、Ge和Se的最佳浓度对蛋白质、多糖、虫草酸和氨基酸的生物合成有积极作用。小鼠血液中谷胱甘肽过氧化物酶（GSH - Px）和超氧化物歧化酶（SOD）的活性分别比对照组高3.72±0.15%和28.74±2.53%，丙二醛（MDA）含量比对照组低41.01±3.66%。
