重评人参根中三醇型人参皂苷 Re、Rf、Rg2 和 20-O-β-D-吡喃葡萄糖基-Rf 的理化性质和 NMR 数据。
Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots.
机构信息
Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.
出版信息
J Ginseng Res. 2014 Apr;38(2):116-22. doi: 10.1016/j.jgr.2013.10.002. Epub 2013 Dec 17.
Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as (1)H-(1)H correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published (1)H- and (13)C-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.
人参根用甲醇水溶液提取，提取物混悬于水中，依次用乙酸乙酯和正丁醇萃取。正丁醇部分经柱色谱分离得到四种纯化的三醇人参皂苷：人参皂苷 Re、Rf、Rg2 和 20-葡糖基-Rf。测定了这些人参皂苷的理化和光谱特征，并与文献报道进行了比较。进行光谱分析时，采用二维核磁共振（NMR）方法，如（1）H-（1）H 相关谱、核 Overhauser 效应谱、异核单量子相关谱和异核多键相关谱，以确定准确的峰归属。发现先前发表的（1）H-和（13）C-NMR 图谱中的一些峰归属不准确。本研究首次报道了 20-葡糖基-Rf 的完整 NMR 归属。
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