J Biomed Nanotechnol. 2014 Jun;10(6):911-31. doi: 10.1166/jbn.2014.1783.
A new composition of gelatin/bioactive-glass/silver nanoparticle was synthesized and employed to prepare antibacterial macroporous scaffolds with potential applications in bone tissue engineering. A set of macroporous nanocomposite scaffolds were developed from an aqueous solution of gelatin by freeze-drying and crosslinking using genipin at ambient temperature. Silver nanoparticles were successfully synthesized in situ in gelatin solution by heat treatment reduction as a simple and "green" method in which gelatin acted as a natural reducing and stabilizing agent. The effect of the incorporation of the bioactive-glass and the silver nanoparticle concentration on the physicochemical properties of the scaffolds, such as the gel fraction, porosity, in vitro enzyme degradation, morphology, and swelling behavior was studied. Furthermore, the in vitro viability of human mesenchymal stem cells (hMSC) and the antibacterial activity against gram-negative Escherichia coli and gram-positive Staphylococcus aureus were tested on the scaffolds. It was found that upon the addition of silver nanoparticles the porosity, pore size, swelling, and antibacterial properties were enhanced. The silver nanoparticles increased the in vitro enzyme degradation in samples without bioactive-glass; however, the degradation was remarkably reduced by addition of bioactive-glass. In addition, formation of apatite particles, the main inorganic constituent of the bone, on the surface of the bioactive-glass containing scaffolds were confirmed after immersion in simulated body fluid (SBF). The viability of hMSC on the scaffold suggested that gelatin/bioactive-glass/nanosilver scaffolds can be used as an antibacterial scaffolds.
一种新型的明胶/生物活性玻璃/银纳米粒子的组合物被合成，并被用于制备具有潜在应用于骨组织工程的抗菌大孔支架。通过在环境温度下使用京尼平进行冷冻干燥和交联，从明胶的水溶液中开发出了一组大孔纳米复合材料支架。通过热处理还原成功地原位合成了银纳米粒子，这是一种简单而“绿色”的方法，其中明胶充当天然的还原和稳定剂。研究了生物活性玻璃的掺入和银纳米粒子浓度对支架的物理化学性质的影响，如凝胶分数、孔隙率、体外酶降解、形态和溶胀行为。此外，还在支架上测试了人骨髓间充质干细胞（hMSC）的体外活力和对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抗菌活性。结果发现，加入银纳米粒子后，孔隙率、孔径、溶胀和抗菌性能得到了提高。银纳米粒子增加了不含生物活性玻璃的样品的体外酶降解；然而，加入生物活性玻璃后，降解显著减少。此外，在模拟体液（SBF）中浸泡后，确认了含有生物活性玻璃的支架表面形成了磷灰石颗粒，这是骨骼的主要无机成分。支架上 hMSC 的活力表明，明胶/生物活性玻璃/纳米银支架可用作抗菌支架。
