a Department of Ceramic Engineering , National Institute of Technology , Rourkela , Odisha , India.
J Biomater Sci Polym Ed. 2019 May;30(7):561-579. doi: 10.1080/09205063.2019.1587697. Epub 2019 Mar 26.
Macroporous composite scaffolds comprising of gelatin and glass ceramic has been fabricated and characterized for bone tissue engineering applications. Gelatin scaffold with varying glass-ceramic content was fabricated using lyophilization technique. The microstructure, compressive strength, bioactivity, biodegradation and biocompatibility of the fabricated scaffolds were evaluated. The scaffolds presented macroporous pore size with porosity varying from 79 to 84%. The compressive strength was enhanced by glass ceramic addition and the scaffolds exhibited strength in the range of 1.9 to 5.7 MPa. The obtained strength and porosity was in the range of cancellous bone. The dissolution of gelatin scaffolds was optimized by an additional in situ glutaraldehyde crosslinking step and further by glass-ceramic addition. The composite scaffolds showed good apatite-forming ability in vitro. Biocompatibility and osteogenic ability of the scaffolds were analyzed in vitro by cell adhesion study, alkaline phosphatase activity and Alizarin S staining. The obtained results revealed the composite scaffolds possessed enhanced osteogenic ability and good cell adhesion properties. The developed scaffold is a prospective candidate as a biomaterial for bone tissue engineering.
已制备并表征了包含明胶和玻璃陶瓷的大孔复合支架，用于骨组织工程应用。使用冷冻干燥技术制备了具有不同玻璃陶瓷含量的明胶支架。评估了所制备支架的微观结构、压缩强度、生物活性、生物降解性和生物相容性。支架呈现出大孔孔径，孔隙率从 79%到 84%不等。玻璃陶瓷的添加提高了压缩强度，支架的强度范围为 1.9 到 5.7 MPa。获得的强度和孔隙率在松质骨的范围内。通过额外的原位戊二醛交联步骤和玻璃陶瓷的添加优化了明胶支架的溶解。复合支架在体外表现出良好的磷灰石形成能力。通过细胞黏附研究、碱性磷酸酶活性和茜素 S 染色对支架的生物相容性和成骨能力进行了体外分析。得到的结果表明，复合支架具有增强的成骨能力和良好的细胞黏附特性。开发的支架是骨组织工程生物材料的有前途的候选者。
