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将一种血管内皮生长因子模拟肽“点击”固定在脱细胞内皮细胞外基质上以增强血管生成。

"Click" immobilization of a VEGF-mimetic peptide on decellularized endothelial extracellular matrix to enhance angiogenesis.

作者信息

Wang Lin, Zhao Meirong, Li Siheng, Erasquin Uriel J, Wang Hao, Ren Li, Chen Changyi, Wang Yingjun, Cai Chengzhi

机构信息

Department of Chemistry, University of Houston , Houston, Texas 77204, United States.

出版信息

ACS Appl Mater Interfaces. 2014 Jun 11;6(11):8401-6. doi: 10.1021/am501309d. Epub 2014 May 1.

DOI:10.1021/am501309d
PMID:24749832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4059262/
Abstract

We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment. Moreover, the DC-ECM can be modified with a peptide (QK) mimicking vascular endothelial growth factor without apparently compromising its integrity. The modification was achieved through metabolic incorporation of a "clickable" handle to DC-ECM followed by rapid attachment of the QK peptide with an azido tag using copper-catalyzed click reaction. The attachment of the QK peptide on to DC-ECM in this way further enhanced the angiogenic responses (formation of branched tubular networks) of endothelial cells.

摘要

我们表明，在底物表面包被去细胞外基质（DC-ECM）是生成模拟天然细胞外基质环境平台的有效方法。此外，DC-ECM可用模拟血管内皮生长因子的肽（QK）进行修饰，而不会明显损害其完整性。修饰是通过将一个“可点击”手柄代谢掺入DC-ECM，然后使用铜催化的点击反应将带有叠氮标签的QK肽快速连接来实现的。以这种方式将QK肽连接到DC-ECM上进一步增强了内皮细胞的血管生成反应（形成分支管状网络）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/e3d843936bad/am-2014-01309d_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/9c85c05f125c/am-2014-01309d_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/10b9acc04f1b/am-2014-01309d_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/f3d496d08ccc/am-2014-01309d_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/e3d843936bad/am-2014-01309d_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/9c85c05f125c/am-2014-01309d_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/10b9acc04f1b/am-2014-01309d_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/f3d496d08ccc/am-2014-01309d_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f59e/4059262/e3d843936bad/am-2014-01309d_0004.jpg

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