Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands; Vestergaard Frandsen SA, Lausanne, Switzerland.
Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands; Vestergaard Frandsen SA, Lausanne, Switzerland
Am J Trop Med Hyg. 2014 Jun;90(6):1124-32. doi: 10.4269/ajtmh.13-0755. Epub 2014 Apr 21.
Flight behavior of insecticide-resistant and susceptible malaria mosquitoes approaching deltamethrin-treated nets was examined using a wind tunnel. Behavior was linked to resulting health status (dead or alive) using comparisons between outcomes from free-flight assays and standard World Health Organization (WHO) bioassays. There was no difference in response time, latency time to reach the net, or spatial distribution in the wind tunnel between treatments. Unaffected resistant mosquitoes spent less time close to (< 30 cm) treated nets. Nettings that caused high knockdown or mortality in standard WHO assays evoked significantly less mortality in the wind tunnel; there was no excitorepellent effect in mosquitoes making contact with the nettings in free flight. This study shows a new approach to understanding mosquito behavior near insecticidal nets. The methodology links free-flight behavior to mosquito health status on exposure to nets. The results suggest that behavioral assays can provide important insights for evaluation of insecticidal effects on disease vectors.
使用风洞研究了抗药性和敏感性疟蚊接近拟除虫菊酯处理过的蚊帐时的飞行行为。使用自由飞行试验和世界卫生组织（WHO）标准生物测定结果之间的比较，将行为与最终健康状况（死亡或存活）联系起来。在处理之间，对反应时间、到达网的潜伏期或风洞中空间分布没有差异。未受影响的抗性蚊子在靠近（<30 厘米）处理过的网的时间较少。在标准 WHO 测定中引起高击倒或死亡率的网引起的死亡率在风洞中显著降低；在自由飞行中与网接触的蚊子没有兴奋驱避作用。这项研究展示了一种理解蚊子在防虫网附近行为的新方法。该方法将自由飞行行为与蚊子暴露于网时的健康状况联系起来。结果表明，行为测定可以为评估杀虫剂对病媒的影响提供重要的见解。
