Khampieng Thitikan, Brikshavana Pasakorn, Supaphol Pitt
a Center of Excellence on Petrochemical and Materials Technology (PETROMAT), The Petroleum Petrochemical College, Chulalongkorn University , Bangkok 10330 , Thailand.
J Biomater Sci Polym Ed. 2014;25(8):826-42. doi: 10.1080/09205063.2014.910154. Epub 2014 Apr 23.
Silver nanoparticle (nAg)-embedded poly(vinyl pyrrolidone) (PVP) hydrogels, to be used as antibacterial wound dressings, were prepared by γ-irradiation at various doses: 25, 35, and 45 kGy. The formation and characteristics of the silver nanoparticles were investigated with a UV-vis spectrophotometer, transmission electron microscopy, and scanning electron microscopy with energy-dispersive X-ray. The hydrogels were characterized for physical and biological properties. Based on the antibacterial determination, the 1 and 5 mM nAg-embedded PVP hydrogels were effective, with 99.99% bactericidal activity at 12 and 6 h, respectively. The indirect cytotoxicity evaluation based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated that both the neat and the nAg-embedded PVP hydrogels were non-toxic to mouse fibroblasts (L929). The 5 mM nAg-embedded PVP hydrogels not only provided a clean, moist environment for wound healing, but also effectively prevented bacterial infection and enhanced wound recovery.
通过γ射线在25、35和45千戈瑞等不同剂量下辐照制备了嵌入银纳米颗粒（nAg）的聚（乙烯基吡咯烷酮）（PVP）水凝胶，用作抗菌伤口敷料。用紫外可见分光光度计、透射电子显微镜和带能谱仪的扫描电子显微镜研究了银纳米颗粒的形成和特性。对水凝胶的物理和生物学性质进行了表征。基于抗菌测定，1和5毫摩尔嵌入nAg的PVP水凝胶有效，分别在12小时和6小时具有99.99%的杀菌活性。基于3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定的间接细胞毒性评估表明，纯PVP水凝胶和嵌入nAg的PVP水凝胶对小鼠成纤维细胞（L929）均无毒。5毫摩尔嵌入nAg的PVP水凝胶不仅为伤口愈合提供了清洁、湿润的环境，还有效防止了细菌感染并促进了伤口恢复。
