Heikinheimo K, Hormia M, Stenman G, Virtanen I, Happonen R P
Department of Pathology, University of Helsinki, Finland.
J Oral Pathol Med. 1989 May;18(5):264-73. doi: 10.1111/j.1600-0714.1989.tb00395.x.
Monoclonal antibodies (Mab) were used to study the expression of cytokeratins and vimentin in various histological types of ameloblastoma and in human fetal tooth germ. The ameloblastoma and the tooth germ epithelia showed characteristics of both simple glandular and stratified squamous epithelial cells. Cytokeratin No. 18 was detected focally in most ameloblastomas studied but not in fetal odontogenic epithelia. Cytokeratins Nos. 8 and 19 were expressed in all epithelial elements of ameloblastomas and tooth germs. Only two tumors showed focally characteristics of keratinizing epithelia also seen in dental lamina but not in the enamel organ. All tumors except the granular cell ameloblastoma showed a variable coexpression of vimentin and cytokeratins in their neoplastic epithelia. A similar coexpression was detected in the stellate reticulum cells of the developing tooth. Ameloblastoma and human tooth germ epithelia share complex pattern of cytokeratin polypeptides together with coexpression of vimentin. The results strongly support the theory that ameloblastomas are of odontogenic origin and not direct derivatives of basal cells of oral epithelium or epidermis.
单克隆抗体（Mab）被用于研究细胞角蛋白和波形蛋白在各种组织学类型的成釉细胞瘤以及人胎儿牙胚中的表达。成釉细胞瘤和牙胚上皮显示出单层腺上皮细胞和复层鳞状上皮细胞的特征。在大多数所研究的成釉细胞瘤中可局灶性检测到细胞角蛋白18，但在胎儿牙源性上皮中未检测到。细胞角蛋白8和19在成釉细胞瘤和牙胚的所有上皮成分中均有表达。仅两个肿瘤显示出在牙板中可见但在成釉器中未见的角化上皮的局灶性特征。除颗粒细胞成釉细胞瘤外，所有肿瘤的肿瘤上皮中波形蛋白和细胞角蛋白均呈不同程度的共表达。在发育中的牙齿的星网状细胞中也检测到类似的共表达。成釉细胞瘤和人牙胚上皮共同具有复杂的细胞角蛋白多肽模式以及波形蛋白的共表达。这些结果有力地支持了成釉细胞瘤起源于牙源性而非口腔上皮或表皮基底细胞的直接衍生物这一理论。
