Arai K, Yasuda J, Sasaki T, Yonemasu K
Central Laboratory for Clinical Investigation, Osaka University Medical School, Japan.
Jpn J Med Sci Biol. 1988 Oct-Dec;41(5-6):189-96. doi: 10.7883/yoken1952.41.189.
Antibodies against heat-aggregated human IgG (AHG) were raised in rabbits and those against immune complex (IC) of tetanus toxoid and its human antitoxin (T/aT) in rabbits and mice. These antisera were analyzed by the Ouchterlony double diffusion technique against native monomeric human IgG (NHG), AHG and chemically linked human IgG (poly-G). The appearance of neoantigens was demonstrated with human IgG bound to its corresponding antigen or denaturated nonspecifically. The results are indicative of the existence of a common determinant shared with AHG and T/aT, in addition to those of their own.
在兔体内产生了抗热聚集人IgG（AHG）的抗体，在兔和小鼠体内产生了抗破伤风类毒素及其人抗毒素（T/aT）免疫复合物（IC）的抗体。采用双向琼脂扩散技术，用天然单体人IgG（NHG）、AHG和化学连接的人IgG（poly-G）对这些抗血清进行分析。通过人IgG与其相应抗原结合或非特异性变性，证明了新抗原的出现。结果表明，除了AHG和T/aT自身的决定簇外，还存在与它们共有的共同决定簇。
