[In vitro culture of melanocyte-bearing epidermis and its application in the treatment of vitiligo and the stable leukodermas].

Skin specimens were cultured in vitro in a attempt to reproduce epidermal sheets bearing melanocytes. After exploring different parameters and culture conditions, 16 out of 45 samples yielded pigmented epidermal membranes with melanocytes. This epidermis bearing melanocytes was transplanted to a patient with segmental vitiligo and a satisfactory repigmentation was achieved. The factors contributing to the development of this pigmented in vitro cultured epidermis, and the potential of this technology for treating refractory but stable forms of vitiligo, are discussed.