Suvanprakorn P, Dee-Ananlap S, Pongsomboon C, Klaus S N
J Am Acad Dermatol. 1985 Dec;13(6):968-74. doi: 10.1016/s0190-9622(85)70247-2.
Patients with three types of leukoderma--vitiligo, idiopathic guttate hypomelanosis, and postinflammatory leukoderma--had successful repigmentation after transplantation of autologous melanocytes. The procedure was performed easily by producing blisters on normal skin and on depigmented lesions. Blisters were produced by suction or by freezing with liquid nitrogen. The roof of the blister from donor skin was grafted to the raw surface of the recipient site. Repigmentation was visible within 7 to 14 days. Direct immunofluorescence staining with bullous pemphigoid antibodies suggested that the separation of the epidermis from the dermis occurs within the lamina lucida. Histochemical studies confirmed the absence of dopa-positive cells in the areas of leukoderma prior to grafting. Melanocytes were present in the successful grafts.
患有三种类型白斑病（白癜风、特发性点滴状色素减退症和炎症后白斑病）的患者在自体黑素细胞移植后成功实现了色素再生。该手术通过在正常皮肤和色素脱失性皮损上产生水疱来轻松完成。水疱通过抽吸或液氮冷冻产生。将供体皮肤水疱的顶部移植到受体部位的创面上。色素再生在7至14天内可见。用大疱性类天疱疮抗体进行直接免疫荧光染色表明，表皮与真皮的分离发生在透明层内。组织化学研究证实，移植前白斑区域不存在多巴阳性细胞。成功的移植物中存在黑素细胞。
