Jones B E, Cuello A C
Department of Neurology and Neurosurgery, Montreal Neurological Institute, Quebec, Canada.
Neuroscience. 1989;31(1):37-61. doi: 10.1016/0306-4522(89)90029-8.
The afferent input to the basal forebrain cholinergic neurons from the pontomesencephalic tegmentum was examined by retrograde transport of wheatgerm agglutinin-horseradish peroxidase in combination with immunohistochemistry. Multiple tyrosine hydroxylase-, dopamine-beta-hydroxylase-, serotonin- and choline acetyltransferase-immunoreactive fibres were observed in the vicinity of the choline acetyltransferase-immunoreactive cell bodies within the globus pallidus, substantia innominata and magnocellular preoptic nucleus. Micro-injections of horseradish peroxidase-conjugated wheatgerm agglutinin into this area of cholinergic perikarya led to retrograde labelling of a large population of neurons within the pontomesencephalic tegmentum, which included cells in the ventral tegmental area, substantia nigra, retrorubral field, raphe nuclei, reticular formation, pedunculopontine tegmental nucleus, laterodorsal tegmental nucleus, parabrachial nuclei and locus coeruleus nucleus. Of the total population of retrogradely labelled neurons, a significant (approximately 25%) proportion were tyrosine hydroxylase-immunoreactive and found in the ventral tegmental area (A10), the substantia nigra (A9), the retrorubral field (A8), the raphe nuclei (dorsalis, linearis and interfascicularis) and the locus coeruleus nucleus (A6), Another important contingent (approximately 10%) was represented by serotonin neurons of the dorsal raphe nucleus (B7), the central superior nucleus (B8) and ventral tegmentum (B9). A small proportion (less than 1%) was represented by cholinergic neurons of the pedunculopontine (Ch5) and laterodorsal (Ch6) tegmental nuclei. These results demonstrate that pontomesencephalic monoamine neurons project in large numbers up to the basal forebrain cholinergic neurons and may represent a major component of the ventral tegmental pathway that forms the extra-thalamic relay from the brainstem through the basal forebrain to the cerebral cortex.
通过结合免疫组织化学的小麦胚凝集素 - 辣根过氧化物酶逆行运输，研究了脑桥中脑被盖向基底前脑胆碱能神经元的传入输入。在苍白球、无名质和大细胞视前核内胆碱乙酰转移酶免疫反应性细胞体附近观察到多条酪氨酸羟化酶、多巴胺β - 羟化酶、5 - 羟色胺和胆碱乙酰转移酶免疫反应性纤维。将辣根过氧化物酶偶联的小麦胚凝集素微量注射到胆碱能核周体的这个区域，导致脑桥中脑被盖内大量神经元的逆行标记，其中包括腹侧被盖区、黑质、红核后区、中缝核、网状结构、脚桥被盖核、外侧背侧被盖核、臂旁核和蓝斑核中的细胞。在逆行标记的神经元总数中，相当大比例（约25%）是酪氨酸羟化酶免疫反应性的，见于腹侧被盖区（A10）、黑质（A9）、红核后区（A8）、中缝核（背侧、线性和束间）和蓝斑核（A6）。另一个重要群体（约10%）由背侧中缝核（B7）、中央上核（B8）和腹侧被盖区（B9）的5 - 羟色胺神经元代表。一小部分（不到1%）由脚桥（Ch5）和外侧背侧（Ch6）被盖核的胆碱能神经元代表。这些结果表明，脑桥中脑单胺能神经元大量投射到基底前脑胆碱能神经元，可能代表腹侧被盖通路的主要成分，该通路形成从脑干经基底前脑到大脑皮层的丘脑外中继。
