Hanazono K, Nishimori T, Fukumoto S, Kawamura Y, Endo Y, Kadosawa T, Uchide T
Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Vet Comp Oncol. 2016 Sep;14(3):263-9. doi: 10.1111/vco.12095. Epub 2014 Apr 24.
Transitional cell carcinoma (TCC) is a urinary bladder tumour associated with high mortality in dogs. In this study, we investigated the feasibility of using p63, Ki67 or β-catenin as a clinical marker for predicting biological behaviour and prognosis in canine TCC. Expression levels of these proteins in TCC (n = 25), polypoid cystitis (n = 5) and normal urinary bladder (n = 5) were scored after immunohistochemical staining. The staining scores for p63 (P < 0.01) and β-catenin (P < 0.05) in TCC were significantly lower than those in normal urinary bladder and polypoid cystitis. In contrast, Ki67 (P < 0.01) staining scores in TCC were significantly higher than those in normal urinary bladder and polypoid cystitis. In TCC, low p63 expression was significantly related to the presence of vessel invasion (P < 0.05) and metastasis (P < 0.01) as well as short survival time (P < 0.05). These findings show that p63 could be a reliable marker for predicting prognosis in canine TCC.
移行细胞癌（TCC）是一种与犬类高死亡率相关的膀胱肿瘤。在本研究中，我们调查了使用p63、Ki67或β-连环蛋白作为预测犬TCC生物学行为和预后的临床标志物的可行性。免疫组织化学染色后，对这些蛋白在TCC（n = 25）、息肉样膀胱炎（n = 5）和正常膀胱（n = 5）中的表达水平进行评分。TCC中p63（P < 0.01）和β-连环蛋白（P < 0.05）的染色评分显著低于正常膀胱和息肉样膀胱炎。相反，TCC中Ki67（P < 0.01）染色评分显著高于正常膀胱和息肉样膀胱炎。在TCC中，低p63表达与血管侵犯（P < 0.05）、转移（P < 0.01）以及生存时间短（P < 0.05）显著相关。这些发现表明，p63可能是预测犬TCC预后的可靠标志物。
