使用基质辅助激光解吸电离成像质谱（MALDI-IMS）和超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UHPLC-ESI-QTOF）分析异种移植瘤的脂质组。
Analysis of the lipidome of xenografts using MALDI-IMS and UHPLC-ESI-QTOF.
作者信息
Fernández Roberto, Lage Sergio, Abad-García Beatriz, Barceló-Coblijn Gwendolyn, Terés Silvia, López Daniel H, Guardiola-Serrano Francisca, Martín M Laura, Escribá Pablo V, Fernández José A
机构信息
Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena s/n, 48940, Leioa, Spain.
出版信息
J Am Soc Mass Spectrom. 2014 Jul;25(7):1237-46. doi: 10.1007/s13361-014-0882-3. Epub 2014 Apr 24.
Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and to test new drug candidates. Among the parameters analyzed are the variations in the lipid composition, as they are good indicators of changes in the cellular metabolism. Here, we present a study on the distribution of lipids in xenografts of NCI-H1975 human lung cancer cells, using MALDI imaging mass spectrometry and UHPLC-ESI-QTOF. The identification of lipids directly from the tissue by MALDI was aided by the comparison with identification using ESI ionization in lipid extracts from the same xenografts. Lipids belonging to PCs, PIs, SMs, DAG, TAG, PS, PA, and PG classes were identified and their distribution over the xenograft was determined. Three areas were identified in the xenograft, corresponding to cells in different metabolic stages and to a layer of adipose tissue that covers the xenograft.
免疫缺陷小鼠体内的人肿瘤异种移植是研究癌症发展和测试新候选药物的一种非常流行的模型。所分析的参数包括脂质组成的变化，因为它们是细胞代谢变化的良好指标。在此，我们使用基质辅助激光解吸电离成像质谱（MALDI成像质谱）和超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UHPLC-ESI-QTOF），对NCI-H1975人肺癌细胞异种移植瘤中的脂质分布进行了研究。通过与来自相同异种移植瘤脂质提取物中使用电喷雾电离（ESI）进行的鉴定相比较，辅助MALDI直接从组织中鉴定脂质。鉴定出了属于磷脂酰胆碱（PC）、磷脂酰肌醇（PI）、鞘磷脂（SM）、二酰基甘油（DAG）、三酰基甘油（TAG）、磷脂酰丝氨酸（PS）、磷脂酸（PA）和磷脂酰甘油（PG）类别的脂质，并确定了它们在异种移植瘤中的分布。在异种移植瘤中确定了三个区域，分别对应处于不同代谢阶段的细胞以及覆盖异种移植瘤的一层脂肪组织。
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