• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.禽成髓细胞瘤病毒DNA聚合酶进行DNA合成的模板特异性条件观察
Nucleic Acids Res. 1976 Jun;3(6):1473-86. doi: 10.1093/nar/3.6.1473.
2
Spermine stimulates RNA-dependent reverse transcriptase activity.精胺刺激RNA依赖性逆转录酶活性。
Biochem Int. 1989 Jul;19(1):67-76.
3
Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.禽成髓细胞瘤病毒逆转录酶与禽成髓细胞瘤病毒RNA的相互作用机制
J Virol. 1976 Apr;18(1):26-33. doi: 10.1128/JVI.18.1.26-33.1976.
4
Utilization of mismatched initiator termini by avian myeloblastosis virus DNA polymerase.禽成髓细胞瘤病毒DNA聚合酶对错配起始末端的利用
Biochim Biophys Acta. 1979 Dec 17;565(2):225-30. doi: 10.1016/0005-2787(79)90200-4.
5
[DNA synthesis on the heterogeneous nuclear RNA template catalysed by DNA polymerase of avian myeloblastosis virus].[禽成髓细胞瘤病毒DNA聚合酶催化的异质核RNA模板上的DNA合成]
Mol Biol (Mosk). 1975 Sep-Oct;9(5):768-74.
6
RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。VI. 禽成髓细胞瘤病毒聚合酶的持续作用模式。
J Virol. 1976 Sep;19(3):932-9. doi: 10.1128/JVI.19.3.932-939.1976.
7
Processive nature of reverse transcription by avian myeloblastosis virus deoxyribonucleic acid polymerase.禽成髓细胞瘤病毒脱氧核糖核酸聚合酶逆转录的持续合成特性。
Biochemistry. 1980 Jan 22;19(2):301-6. doi: 10.1021/bi00543a008.
8
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.禽成髓细胞瘤病毒逆转录酶用于合成反互补DNA时所使用的RNA引物。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1316-20. doi: 10.1073/pnas.77.3.1316.
9
Model RNA-directed DNA synthesis by avian myeloblastosis virus DNA polymerase and its associated RNase H.禽成髓细胞瘤病毒DNA聚合酶及其相关核糖核酸酶H介导的RNA指导的DNA合成模型
Biochemistry. 1979 Jul 24;18(15):3210-9. doi: 10.1021/bi00582a004.
10
Apparent allosterism by avian myeloblastosis virus reverse transcriptase and E. coli DNA polymerase I.禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I的明显变构效应
Nucleic Acids Res. 1979 Mar;6(3):1189-201. doi: 10.1093/nar/6.3.1189.

引用本文的文献

1
Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification.Chum-RNA无需PCR扩增即可从单细胞数量的mRNA制备高质量的cDNA文库。
Nucleic Acids Res. 2008 Sep;36(15):e92. doi: 10.1093/nar/gkn420. Epub 2008 Jul 4.
2
On the early emergence of reverse transcription: theoretical basis and experimental evidence.关于逆转录的早期出现：理论基础与实验证据。
J Mol Evol. 1992 Dec;35(6):524-36. doi: 10.1007/BF00160213.
3
DNA endonucleases associated with the avian myeloblastosis virus DNA polymerase.与禽成髓细胞瘤病毒DNA聚合酶相关的DNA核酸内切酶。
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2659-63. doi: 10.1073/pnas.76.6.2659.
4
Reverse transcriptase-associated RNase H activity. II. Inhibition by natural and synthetic RNA.逆转录酶相关的核糖核酸酶H活性。II. 天然和合成RNA的抑制作用
J Virol. 1978 Sep;27(3):576-81. doi: 10.1128/JVI.27.3.576-581.1978.
5
The effect of magnesium and manganese ions on the structure and template activity for reverse transcriptase of polyribocytidylate and its 2'-0-methyl derivative.镁离子和锰离子对聚核糖胞苷酸及其2'-O-甲基衍生物的结构和逆转录酶模板活性的影响。
Nucleic Acids Res. 1977 Oct;4(10):3589-97. doi: 10.1093/nar/4.10.3589.
6
Specific inhibition of DNA polymerase-associated RNase H by DNA.DNA对DNA聚合酶相关核糖核酸酶H的特异性抑制作用。
J Virol. 1977 Apr;22(1):243-6. doi: 10.1128/JVI.22.1.243-246.1977.

本文引用的文献

1
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.脱氧核糖核酸的酶促合成。IX. T2噬菌体感染大肠杆菌后形成的聚合酶：一种新酶。
J Biol Chem. 1962 Feb;237:519-25.
2
Conformational aspects and reactivity of DNA. Effects of manganese and magnesium ions on interaction with DNA.DNA的构象方面与反应活性。锰离子和镁离子对与DNA相互作用的影响。
Eur J Biochem. 1972 Sep 25;29(3):528-36. doi: 10.1111/j.1432-1033.1972.tb02018.x.
3
A new sensitive method for detecting polyadenylate in viral and other ribonucleic acids using Escherichia coli deoxyribonucleic acid polymerase I.一种使用大肠杆菌脱氧核糖核酸聚合酶I检测病毒及其他核糖核酸中聚腺苷酸的新灵敏方法。
J Biol Chem. 1974 Nov 25;249(22):7373-6.
4
Replication of initiated polyriboadenylic acid by mammalian low molecular weight deoxyribonucleic acid polymerase.哺乳动物低分子量脱氧核糖核酸聚合酶对起始的聚核糖核酸的复制
J Biol Chem. 1974 Dec 10;249(23):7441-6.
5
Synthesis of DNA complementary to AMV RNA using E. coli polymerase I.使用大肠杆菌聚合酶I合成与禽成髓细胞瘤病毒RNA互补的DNA
Biochem Biophys Res Commun. 1974 Jan;56(1):247-55. doi: 10.1016/s0006-291x(74)80341-4.
6
Comparative studies on the template-initiator requirements of the chick-embryo DNA polymerase I and the avian-myeloblastosis-virus DNA polymerase.鸡胚DNA聚合酶I与禽成髓细胞瘤病毒DNA聚合酶模板-引发剂需求的比较研究。
Eur J Biochem. 1974 Jan 16;41(2):253-61. doi: 10.1111/j.1432-1033.1974.tb03266.x.
7
Purification of the DNA polymerase of avian myeloblastosis virus.禽成髓细胞瘤病毒DNA聚合酶的纯化
Biochim Biophys Acta. 1971 Sep 24;246(3):365-83. doi: 10.1016/0005-2787(71)90773-8.
8
Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli, and Micrococcus luteus.禽成髓细胞瘤病毒、大肠杆菌和藤黄微球菌的脱氧核糖核酸聚合酶催化的一些反应的比较
Biochemistry. 1972 Feb 15;11(4):621-9. doi: 10.1021/bi00754a025.
9
RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。
J Virol. 1972 Jan;9(1):130-42. doi: 10.1128/JVI.9.1.130-142.1972.
10
Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases.区分RNA肿瘤病毒的逆转录酶与其他已知的DNA聚合酶。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203-6. doi: 10.1073/pnas.68.9.2203.

禽成髓细胞瘤病毒DNA聚合酶进行DNA合成的模板特异性条件观察

Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.

作者信息

Marcus S L, Modak M J

出版信息

Nucleic Acids Res. 1976 Jun;3(6):1473-86. doi: 10.1093/nar/3.6.1473.

DOI:10.1093/nar/3.6.1473
PMID:60740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343006/
Abstract

The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.

摘要

研究了单独添加和组合添加Mg++、Mn++和KCl对禽成髓细胞瘤病毒DNA聚合酶（逆转录酶）利用多种天然和合成模板-引物组合进行DNA引发的DNA合成以及RNA引发的DNA合成速率的影响。发现最佳二价阳离子浓度根据用于指导合成的模板-引物不同而变化高达10倍。向含有最佳二价阳离子浓度的反应混合物中添加KCl会产生对DNA合成的刺激或抑制，这也是模板特异性的。在修饰模板聚（2'-O-甲基胞苷酸）上的DNA合成受到二价阳离子组合的独特刺激。以Mg++作为二价阳离子时，在所测试的所有模板-引物中均观察到与经典米氏底物饱和动力学的偏差。