二甲基亚砜对逆转录酶活性的影响。

Effect of dimethyl sulfoxide on reverse transcriptase activity.

作者信息

Jucá M B, Aoyama H

机构信息

Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brasil.

出版信息

Braz J Med Biol Res. 1995 Mar;28(3):285-90.

PMID:8520520

Abstract

At low concentrations, dimethyl sulfoxide (DMSO) stimulated the avian myeloblastosis virus reverse transcriptase activity. About 40% stimulation was obtained in the presence of 5% (v/v) DMSO, using activated DNA and polyriboadenylic acid (poly(rA)) as templates, and Mg2+ as divalent cation. A similar stimulation by DMSO was observed with Mn2+ for the poly(rA)-dependent reverse transcriptase activity. DMSO at concentrations higher than 15% inhibited the reverse transcriptase reactions, independent of the template-primers used. An exception was detected with the 2'-fluoro analog of poly(rA) as template, where an activation of 100% was found in the presence of 20% DMSO. The stimulation caused by DMSO could be due to a reduction of the apparent Km value for poly(rA) from 9.1 to 3.3 micrograms/ml.

摘要

在低浓度下，二甲基亚砜（DMSO）刺激禽成髓细胞瘤病毒逆转录酶活性。以活化的DNA和聚腺苷酸（poly(rA)）为模板，Mg2+作为二价阳离子，在5%（v/v）DMSO存在的情况下可获得约40%的刺激作用。对于依赖poly(rA)的逆转录酶活性，用Mn2+时观察到DMSO有类似的刺激作用。浓度高于15%的DMSO抑制逆转录酶反应，这与所用的模板引物无关。以poly(rA)的2'-氟类似物作为模板时是个例外，在20% DMSO存在的情况下发现有100%的激活作用。DMSO引起的刺激作用可能是由于poly(rA)的表观Km值从9.1微克/毫升降至3.3微克/毫升。