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[可可毛色二孢菌刺激白木香倍半萜生成的研究]

[Study of production of sesquiterpenes of Aquilaria senensis stimulated by Lasiodiplodia theobromae].

作者信息

Han Xiao-Min, Liang Liang, Zhang Zheng, Li Xiu-Jin, Yang Yun, Meng Hui, Gao Zhi-Hui, Xu Yan-Hong

出版信息

Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):192-6.

Abstract

To investigate the mechanism of agarwood formation in Aquilaria sinensis induced by Lasiodiplodia theobromae, the fermentation liquor of L. theobromae was analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (GC-MS). JAs were detected in the fermentation liquor. The effect of the fermentation liquor on the abundance of sesquiterpenes in the callus of A. sinensis was analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). And the fermentation liquor stimulated alpha-guaiene, alpha-humulene and delta-guaiene biosynthesis in calli. It was inferred that L. theobromae produced JAs, which resulted in a significant increase of sesquiterpenes in A. sinensis.

摘要

为探究由可可毛色二孢菌诱导白木香形成沉香的机制,采用气相色谱-质谱联用仪(GC-MS)对可可毛色二孢菌的发酵液进行了定性和定量分析。在发酵液中检测到了茉莉酸类物质(JAs)。采用固相微萃取-气相色谱-质谱联用仪(SPME-GC-MS)分析了发酵液对白木香愈伤组织中倍半萜含量的影响。结果表明,发酵液刺激了愈伤组织中α-愈创木烯、α-葎草烯和δ-愈创木烯的生物合成。由此推断,可可毛色二孢菌产生的茉莉酸类物质导致了白木香中倍半萜含量的显著增加。

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