Viac J, Su H, Réano A, Kanitakis J, Chardonnet Y, Thivolet J
J Dermatol. 1989 Apr;16(2):98-102. doi: 10.1111/j.1346-8138.1989.tb01229.x.
A monoclonal antibody, ERD5, which recognizes a 29Kd phosphoprotein associated with human estrogen receptor of myometrium was used to study the expression of this protein in normal skin and in cultured human keratinocytes. By indirect immunofluorescence, both in vivo and in vitro keratinocytes showed a variable cytoplasmic staining which increased with cell differentiation. SDS gel electrophoresis of soluble extracts of cultured keratinocytes and normal epidermis showed that P29 was a minor protein. Immunoblot analysis demonstrated that ERD5 strongly reacted only with a 29Kd polypeptide band without any cross-reactivity. These data suggest that keratinocytes might be estrogen sensitive like other cells in which P29 has already been located. The exact role of this protein in the keratinocyte differentiation process and its relationship with estrogen receptors remain to be elucidated.
一种单克隆抗体ERD5，可识别与子宫肌层人雌激素受体相关的29Kd磷蛋白，用于研究该蛋白在正常皮肤和培养的人角质形成细胞中的表达。通过间接免疫荧光法，体内和体外的角质形成细胞均显示出可变的细胞质染色，且随着细胞分化而增加。培养的角质形成细胞和正常表皮可溶性提取物的SDS凝胶电泳显示，P29是一种次要蛋白。免疫印迹分析表明，ERD5仅与一条29Kd多肽带强烈反应，无任何交叉反应。这些数据表明，角质形成细胞可能像其他已定位P29的细胞一样对雌激素敏感。该蛋白在角质形成细胞分化过程中的具体作用及其与雌激素受体的关系仍有待阐明。
