Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China; Shanghai Human Sperm Bank, Shanghai, People's Republic of China.
Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Fertil Steril. 2014 Jul;102(1):54-60.e2. doi: 10.1016/j.fertnstert.2014.03.035. Epub 2014 Apr 24.
To evaluate the potential clinical application of Raman spectroscopy (RS) as a tool that may identify spermatogenesis within human seminiferous tubules.
RS scanning of human testicular tissue at different maturational stages; immunohistochemistry study and metabolomic analysis of nonobstructive azoospermic/obstructive azoospermic testes.
State-owned hospital.
PATIENT(S): Fifty-two patients with clinical indications of nonobstructive azoospermia (NOA) and obstructive azoospermia (OA) who underwent infertility evaluation and treatment.
INTERVENTION(S): None.
MAIN OUTCOME MEASUREMENT(S): Raman spectra of seminiferous tubules, thickness of lamina propria (LP), immunohistochemistry of type I, III, and IV collagens and laminin, metabolites of human testes.
RESULT(S): Tubules of OA patients had spectral intensities below 2,000 (au), while tubules of NOA patients had higher intensities, depending on the degree of spermatogenesis. RS was able to separate samples of NOA and OA testicular tissue with a sensitivity of 90% and specificity of 85.71%. The LP of NOA tubules were thickened and had increased deposition of type I and type III collagens. Gas chromatography-mass spectrometer (GC-MS) detected 12 metabolites that showed significant differences between NOA and OA testes.
CONCLUSION(S): RS can noninvasively distinguish seminiferous tubules with complete and incomplete spermatogenesis and may serve as a novel and potentially useful tool to guide surgeons performing micro-testicular sperm extraction to improve sperm retrieval.
评估拉曼光谱（RS）作为一种可能识别人类曲细精管内精子发生的工具的潜在临床应用。
对不同成熟阶段的人类睾丸组织进行 RS 扫描；对非梗阻性无精子症/梗阻性无精子症睾丸进行免疫组织化学研究和代谢组学分析。
国有医院。
52 名因非梗阻性无精子症（NOA）和梗阻性无精子症（OA）的临床指征而接受不育评估和治疗的患者。
无。
曲细精管的拉曼光谱、固有层（LP）的厚度、I 型、III 型和 IV 型胶原和层粘连蛋白的免疫组织化学、人睾丸的代谢物。
OA 患者的管腔光谱强度低于 2000（au），而 NOA 患者的管腔具有更高的强度，这取决于精子发生的程度。RS 能够以 90%的灵敏度和 85.71%的特异性分离 NOA 和 OA 睾丸组织的样本。NOA 管腔的 LP 增厚，I 型和 III 型胶原沉积增加。气相色谱-质谱联用仪（GC-MS）检测到 12 种代谢物，它们在 NOA 和 OA 睾丸之间表现出显著差异。
RS 可以无创地区分具有完全和不完全精子发生的曲细精管，并且可能作为一种新的、潜在有用的工具，指导外科医生进行微创睾丸精子提取，以提高精子获取率。
