School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia; Kings Park and Botanic Garden, Botanic Gardens and Parks Authority, West Perth, WA 6005, Australia.
School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia; Kings Park and Botanic Garden, Botanic Gardens and Parks Authority, West Perth, WA 6005, Australia.
Trends Plant Sci. 2014 Aug;19(8):529-37. doi: 10.1016/j.tplants.2014.03.011. Epub 2014 Apr 24.
Restoration ecology is a young scientific discipline underpinning improvements in the rapid global expansion of ecological restoration. The application of molecular tools over the past 20 years has made an important contribution to understanding genetic factors influencing ecological restoration success. Here we illustrate how recent advances in next generation sequencing (NGS) methods are revolutionising the practical contribution of genetics to restoration. Novel applications include a dramatically enhanced capacity to measure adaptive variation for optimal seed sourcing, high-throughput assessment and monitoring of natural and restored biological communities aboveground and belowground, and gene expression analysis as a measure of genetic resilience of restored populations. Challenges remain in data generation, handling and analysis, and how best to apply NGS for practical outcomes in restoration.
恢复生态学是一门年轻的科学学科，为生态恢复的快速全球扩展提供了支持。在过去的 20 年中，分子工具的应用为理解影响生态恢复成功的遗传因素做出了重要贡献。在这里，我们说明下一代测序 (NGS) 方法的最新进展如何彻底改变遗传学对恢复的实际贡献。新的应用包括极大地提高了测量最佳种子来源的适应性变异的能力，高通量评估和监测地上和地下的自然和恢复生物群落，以及作为恢复种群遗传弹性的衡量标准的基因表达分析。在数据生成，处理和分析方面以及如何最好地将 NGS 应用于恢复的实际结果方面仍然存在挑战。
